Effects of high cholesterol diet on sperm small RNA expression profile utilizing two different RNA-seq methods

ABSTRACT: Purpose: To reveal the small RNA profiles in the sperm using traitional and PANDORA-seq methods Methods: 3 week old LDLR-/- male mice were fed a high cholesterol or low cholesterol diet for 9 weeks. Sperm RNA was extracted via Trizol. Results: paternal HCD feeding can lead to sperm tsRNA/rsRNA landscape changes that are undetected by traditional RNA-seq

ORGANISM(S): Mus musculus

PROVIDER: GSE251712 | GEO | 2024/10/08

REPOSITORIES: GEO