A genome wide CRISPR/Cas9 screening to identifiy potential embryonic diapause and diapause-like cancer cell survival genes

ABSTRACT: Diapause-like cancer cells represent a fuel for tumor relapse and strategies that leads to their elimination could be helpful in the context of minimal residual disease and tumor relapse. Here, through a genome-wide CRISPR/Cas9 screen performed in mouse embryonic stem cells undergoing diapause, we identify different genes potentially involved in the survival of diapause-like cancer cells. Further validation experiments in cancer cell lines led to the identification of the pathway NRF2-KEAP1, the 1C metabolism and the DNA methyltransferase DNMT1 as pathways involved in the survival of diapause-like cancer cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE251714 | GEO | 2024/02/01

REPOSITORIES: GEO