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Co-expression network analysis using DNA oligonucleotide microarrays of experimentally infected pigs

ABSTRACT: Co-expression network analysis using DNA oligonucleotide microarrays of experimentally infected pigs. The expression profiles of liver from ten experimentally infected pigs were compared with the profiles of liver from five non-infected contol pigs.

ORGANISM(S): Sus scrofa

PROVIDER: GSE25181 | GEO | 2013/01/01

SECONDARY ACCESSION(S): PRJNA134447

REPOSITORIES: GEO

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Co-expression network analysis using DNA oligonucleotide microarrays of experimentally infected pigs

Project description:Co-expression network analysis using DNA oligonucleotide microarrays of experimentally infected pigs. The expression profiles of liver from ten experimentally infected pigs were compared with the profiles of liver from five non-infected contol pigs. The expression profiles of liver from ten experimentally infected pigs were compared with the profiles of liver from five non-infected contol pigs.

2013-01-01 | E-GEOD-25181 | biostudies-arrayexpress

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Global transcriptional profiling in porcine liver and lymph node tissues during acute pleuropneumoni

Project description:Expression profiles of porcine liver and tracheobronchial lung lymph node tissues were studied to identify genes being significantly affected by bacterial infection of the lungs. Samples were taken from liver and tracheobronchial lung lymph node tissues in pigs experimentally infected with A. pleuropneumoniae, serotype 5b, and from healthy non-inoculated control pigs from the same herd. Samples were investigated for changes in gene expression by means of global cDNA microarrays. Expression profiling of samples of liver and lung lymph node tissues from ten pigs challenged with Actinobacillus pleuropneumoniae and from five healthy control pigs was conducted by hybridising all 2x15 samples against a tissue specific common reference consisting of a pool of equal amount of total-RNA from all samples of each tissue type and were balanced with respect to control and challenged animals.

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Hepatic transcriptional profiling of mRNA and microRNA in pigs infected with Actinobacillus pleuropneumoniae

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Project description:In this study we developed a guinea pig oligonucleotide microarray (GPOM) comprising of a total number of 45,220 features including 43,803 valid features from different mammalian species. These features are inclusive of 2971 newly annotated probes corresponding to 344 unique genes of guinea pig. As a case study, we utilized this array to examine the gene expression profile in guinea pig lungs in response to infection with Mycobacterium tuberculosis. Studying the global gene expression profile in guinea pigs allowed identification of the disease signature of pulmonary TB infection represented by several unique genes that are differentially regulated in this model. While, 1344 unique genes exhibited marked up regulation, 1856 genes were significantly down regulated. The newly developed tool not only finds its utility in studying the global gene expression profile associated with vaccination and/or M. tuberculosis infection in this highly useful animal model but would also be immensely useful in identification of new drug targets, testing of therapies, molecular genetic analysis for diseases other than tuberculosis as well. Genotypic Technology designed Custom Cavia porcellus 4x44k Gene Expression Array (Agilent-AMADID-019424) * In order to validate the GPOM developed in this study, we compared the gene expression profile of guinea pig lungs at 10 weeks post- M. tuberculosis infection with respect to that obtained from normal uninfected animals. To address this, guinea pigs were aerosol infected with M. tuberculosis, lungs were harvested at 10 weeks post-infection and RNA obtained from infected lungs was employed for cDNA synthesis and microarray hybridization. The gene expression was compared with the RNA sample obtained from the lungs of normal uninfected guinea pigs.

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