Snorkel-tag Based Affinity Chromatography for Recombinant Extracellular Vesicle Purification

ABSTRACT: Getting insights on tissue or cell specific EV composition in pathophysiological conditions for developing therapeutics, biomarkers and diagnostic tools in modern medicine still remains a major bottleneck. Similarly, lack of standards for EV comparison also hampers progression in the field. Here, we therefore generated a tagged CD81 fusion protein highly enriched in EVs, enabling affinity isolation of EVs from complex matrices in a non-destructive and pure form without disturbing the here tested EV characteristics.

ORGANISM(S): Homo sapiens

PROVIDER: GSE251842 | GEO | 2024/11/22

REPOSITORIES: GEO