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ARABIDOPSIS HOMOLOG OF THRITORAX impact the lateral root development by epigenetic regulation of targets involved in root system architecture.

ABSTRACT: The chemical modifications of histones between others process are important to modify the chromatin structure and permit the access to DNA. The ARABIDOPSIS HOMOLOG OF TRITHORAX1 (ATX1/SDG27) is a known regulator of floral development which encodes a histone methyltransferase (H3K4me). ATX1 is directly involved in the H3K4 trimethylation which is associated with maintenance of transcriptionally active chromatin state. To study the ATX1 role in lateral root development, we performed a root phenotypic analysis of atx1setm mutant, and the transcriptome profiling (using RNA-seq approach). The atx1setm mutant express the catalytically inactive form of the ATX1 protein (that maintains its structural integrity) in the atx1-1 (null mutant) background. Here, we showed that the primary root and lateral root (LR) primordium (LRP) development in the atx1setm mutant are abnormal, and the phenotype is stronger compared to the atx1-1 mutant. We identified ATX1-activated genes involved in the LR development like PEROXIDASE35 (PER35), AGAMOUS LIKE14 (AGL14), HOMEOBOX PROTEIN 21 (ATHB21), KIDARI (KDR) and USUALLY MULTIPLE ACIDS MOVE IN AND OUT TRANSPORTERS 20 (UMAMIT20). Here, we studied PEROXIDASE 35 gene whose expression is promoted by ATX1 activity. Interestingly, in the per35 mutant the primary root growth was greater compared to Wt and the LRP morphogenesis was abnormal with a phenotype like that found in atx1setm mutant. The data suggest that PER35 gene is a new player related to lateral root primordium morphogenesis in Arabidopsis thaliana.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE252067 | GEO | 2025/06/25

REPOSITORIES: GEO

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Project description:Low phosphate concentrations are frequently a constraint for maize growth and development, and therefore, enormous quantities of phosphate fertilizer are expended in maize cultivation, which increases the cost of planting. Low phosphate stress not only increases root biomass but can also cause significant changes in root morphology. Low phosphate availability has been found to favor lateral root growth over primary root growth by dramatically reducing primary root length and increasing lateral root elongation and lateral root density in Arabdopsis. While in our assay when inbred line Q319 subjected to phosphate starvation, The numbers of lateral roots and lateral root primordia were decreased after 6 days of culture in a low phosphate solution (LP) compared to plants grown under normal conditions (sufficient phosphate, SP), and these differences were increased associated with the stress caused by phosphate starvation. However, the growth of primary roots appeared not to be sensitive to low phosphate levels. This is very different to Arabidopsis. To elucidate how low phosphate levels regulate root modifications, especially lateral root development, a transcriptomic analysis of the 1.0-1.5 cm lateral root primordium zone (LRZ) of maize Q319 treated after 2 and 8 days by low phosphate was completed respectively. The present work utilized an Arizona Maize Oligonucleotide array 46K version slides, which contained 46,000 maize 70-mer oligonucleotides designated by TIGR ID, and the sequence information is available at the website of the Maize Oligonucleotide Array Project as the search item representing the >30,000 identifiable unique maize genes (details at http://www.maizearray.org). Keywords: low phosphate, Lateral Root Primordium Zone, maize Two-condition experiment, low phosphate treated lateral root primordium zone of maize root vs. normal cultrued lateral root primordium zone. Biological replicates: 9 control, 9 treated, independently grown and harvested. One replicate per array.

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ARABIDOPSIS HOMOLOG OF THRITORAX impact the lateral root development by epigenetic regulation of targets involved in root system architecture.

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