Project description:Interventions: lesion tissues vs. adjacent tissues of colorectal cancer patients:nil
Primary outcome(s): RNA
Study Design: Factorial
Project description:<p>We used massively parallel, paired-end sequencing of expressed transcripts (RNA-seq) to detect novel gene fusions in short-term cultures of glioma stem-like cells freshly isolated from nine patients carrying primary glioblastoma multiforme (GBM). The culture of primary GBM tumors under serum-free conditions selects cells that retain phenotypes and genotypes closely mirroring primary tumor profiles as compared to serum-cultured glioma cell lines that have largely lost their developmental identities.</p>
Project description:single cell RNA sequencing of freshly isolated mouse BFU-E (burst forming unit-erythroid ) cells cultured for 1, 2, or 3 days with and without 100nM dexamethasone
Project description:Healthy hearts from warm cadavers not usable for heart transplants were identified and tissues were isolated from these. From ventricle tissues, cardiomyocytes were further isolated. Bulk-RNA-sequencing was performed.
Project description:To identify PRMT1 targets in primary cytotrophoblast (CTBs) cells, we performed CUT&RUN-sequencing. CTBs were freshly isolated from 1st trimester human placenta tissues (5.4 to 9wk).
Project description:Antisense and sense probes of noncoding RNA GAS5 were used to perform the RNA pull down experiment on mouse hippocampus tissues. Then the proteins were identified by LC-MS/MS.
