Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile associated with p53R2 in human cancer cells

ABSTRACT: Deregulation of the expression of p53R2, a p53-inducible homologue of the R2 subunit of ribonucleotide reductase, has been found in various human cancer tissues; however, the roles p53R2 plays in cancer progression and malignancy remain controversial. In present design, we aimed to identify changes in gene expression profiles associated with p53R2 in cancer cells. We compared gene expression file of control KB cells with that of p53R2-attenuated KB cells by its specific siRNA by using cDNA microarray technology. We used microarrays to display the global gene expression profile change, after knockdown of p53R2 expression in human oropharyngeal carcinoma KB cells, to find the gene set regulated by p53R2.

ORGANISM(S): Homo sapiens

PROVIDER: GSE25238 | GEO | 2010/11/10

SECONDARY ACCESSION(S): PRJNA134903

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile associated with p53R2 in human cancer cells

Project description:Deregulation of the expression of p53R2, a p53-inducible homologue of the R2 subunit of ribonucleotide reductase, has been found in various human cancer tissues; however, the roles p53R2 plays in cancer progression and malignancy remain controversial. In present design, we aimed to identify changes in gene expression profiles associated with p53R2 in cancer cells. We compared gene expression file of control KB cells with that of p53R2-attenuated KB cells by its specific siRNA by using cDNA microarray technology. We used microarrays to display the global gene expression profile change, after knockdown of p53R2 expression in human oropharyngeal carcinoma KB cells, to find the gene set regulated by p53R2. To compare changes in gene expression profile in human oropharyngea carcinoma KB cells, KB cells were transfected with control siRNA or p53R2 siRNA, 48h later, total RNA and protein were subjected to Q-PCR and Western blot to confirm a significantly decreased p53R2 mRNA and protein, and then these total RNA samples were for microarray analysis. The cDNA microarray data was analyzed to explore gene sets/pathways, which were significantly impacted by knockdown of p53R2.

2010-11-10 | E-GEOD-25238 | biostudies-arrayexpress

Expression data of peri-tumoral tonsillar tissues in oropharyngeal cancer

Project description:Secondary lymphoid organs (SLOs) serve as an interface between tumor cells and immune system as an initial site of antigen presentation which is critical for the development of an effective anti-tumor immune response. We used microarray to analyze the gene expression profile of peri-tumoral tonsillar tissues of oropharyngeal cancer.

2023-04-02 | GSE228432 | GEO

79d3222f-7720-408b-bf5c-1be9bdaa32d6 - samples

Project description:We profile 10 high-grade gliomas patient brain tumor samples by single-cell multiome ATAC + gene expression, using the 10X Chromium technology. 3 sets of fastq are provided for each samples: R1 and R2 for gene expression, R1 and R2 for ATAC-seq as well as index1 and index2 for ATAC-seq.

| EGAD00001008349 | EGA

OV2295(R2)_DATASET - samples

Project description:10x Genomics Single Cell Gene Expression for High grade serous ovarian carcinoma cell line OV2295(R2)

| EGAD00001009144 | EGA

Expression data of tonsills of chronic tonsillitis, and peri-tumoral tonsillar tissues and lymph nodes in oropharyngeal cancer

Project description:Secondary lymphoid organs (SLOs) serve as an interface between tumor cells and immune system as an initial site of antigen presentation which is critical for the development of an effective anti-tumor immune response. We used microarray to analyze the gene expression profile of tonsills from chronic tonsillitis, and peri-tumoral tonsillar tissues and lymph nodes of oropharyngeal cancer.

2023-10-03 | GSE244580 | GEO

caArray_golub-00327: Sanger cell line Affymetrix gene expression project

Project description:The microarray gene expression pattern was studied using 798 different cancer cell lines. The cancer cell lines are obtained from different centers. Annotation information were provided in the supplementary file.

2015-05-16 | GSE68950 | GEO

Whole Genome DNA Methylation and Gene Expression Profiling of Oropharyngeal Cancer Patients in North-Eastern India

Project description:Methylation of CpG Islands within promoter regions of genes has been associated with gene silencing, suggesting loss of tumor suppressor function and tumorigenesis. The northeastern states of India are leaders in the country for cancers of several sites. Almost no reports are available of any DNA methylation biomarker for oropharyngeal cancers of the northeastern states. The present study aimed to identify targets of CpG hypermethylation and hypomethylation in oropharyngeal cancer prevalent in northeast India. Using Illumina Infinium Human Methylation 450K microarray platform a genome wide screening has been done for differentially methylated genes, including genes not previously implicated in carcinogenesis. Differential gene expression correlated to their methylation status was elaborated using transcriptome profiling. The new genes identified may be used to develop promising panels of DNA Methylation biomarkers for oropharyngeal screening and detection at a very early stage.

2020-09-25 | GSE136704 | GEO

Homo sapiens

Project description:Gene expression profiling of altered NF-kB p65 ser536 phosphorylation in prostate cancer cells

| PRJNA267011 | ENA

Regulation of TROP2 expression in breast cancer

Project description:Expression of genes highly correlated with TACSTD2 expression in breast tumors and corresponding patient-derived xenografts (PDXs) and found that 29 genes were overlapping between in 18 breast cancer samples and matching PDXs. Among these genes, TACSTD2 expression strongly correlated with expression of epithelial marker CDH1 (E-Cadherin) (r2=0.823; p<0.001). Expression of TACSTD2 was then compared to expression of CDH1 in breast cancer cell lines from The Cancer Cell Line Encyclopedia (CCLE) (N = 51) and The Library of Integrated Network-Based Cellular Signatures (LINCS) (N = 35). There was also a significant correlation between TACSTD2 and CDH1 in both CCLE (r2=0.564, p < 0.001) and LINCS (r2= 0.755; p < 0.001).

2023-08-21 | GSE235812 | GEO

Deficiency in tumor suppressor p53 is required for doxorubicin induced transcriptional upregulation of NF-kB target genes in human breast cancer

Project description:NF-kB has been linked to doxorubicin-based chemotherapy resistance in breast cancer patients. NF-kB nuclear translocation and DNA binding in doxorubicin treated-breast cancer cells have been extensively examined, however its functional consequences in terms the spectrum of NF-kB -dependent genes expressed and, thus, the impact on tumour cell behaviour are unclear. We hypothesized that NF-kB gene expression profile induced by doxorubicin might be different among breast cancer cells and tumors. Doxorubicin treatment in the p53-mutated MDA-MB-231 cells resulted in NF-kB driven-gene transcription demonstrated by gene expression microarrays. Selected genes (ICAM-1, CXCL1, IL8) related with invasion, metastasis and chemoresistance expression were confirmed by RT-PCR in a subset of additional doxorubicin-treated cells and fresh primary human breast tumors. In both systems, p53-deficient background correlated with the activation of these NF-kB targeted genes. Overexpression of p53WT in the mutant p53 MDA-MB-231 cells impaired NF-kB driven transcription induced by doxorubicin. Moreover, tumors with a p53 deficient background and nuclear NF-kB /p65 expression correlated with reduced disease free-survival. This study supports that tumor molecular profiles for doxorubicin driven NF-kB-response are likely to exist. A link between p53 deficiency and the presence of active transcriptionally NF-kB could favour an aggressive behaviour and might have implications for doxorubicin-based chemotherapy in breast tumors exhibiting aberrant p53 activity

2013-12-31 | GSE47013 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data