Microglia-astrocyte crosstalk regulates synapse remodeling via Wnt signaling

ABSTRACT: Unilateral whisker lesioning at age P4 leads to removal of thalamocortical synapses in layer IV of the barrel cortex over the course of the next 6 days. Synapse removal is mediated by microglia and induced by CX3CL1-CX3CR1 signaling between neurons and microglia. Downstream of CX3CL1-CX3CR1 activation, microglia induce astrocyte morphological changes. To identify molecular crosstalk between astrocytes and microglia, we performed translating ribosome affinty purification followed by RNA sequencing (TRAP-Seq).

ORGANISM(S): Mus musculus

PROVIDER: GSE252628 | GEO | 2025/08/07

REPOSITORIES: GEO