Assessing chromatin accessibility in maize using ATAC-seq

ABSTRACT: The depiction of maize chromatin architecture using Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq) provides great opportunities to investigate cis-regulatory elements, which is crucial for crop improvement. We demonstrate a streamlined ATAC-seq protocol for maize in which nuclei purification can be achieved without cell sorting, and using only a standard bench-top centrifuge. Our protocol, coupled with the bioinformatic analysis, provides a precise and efficient assessment of the maize chromatin landscape.

ORGANISM(S): Zea mays

PROVIDER: GSE252638 | GEO | 2024/01/10

REPOSITORIES: GEO