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RNA-Seq analysis of WT and Phlpp1-deficient alveolar macrophages in bleomycin-induced PF model

ABSTRACT: The purpose of this study is to detect differentially expressed genes in wild-type (WT) and Phlpp1-deficient alveolar macrophages (AMs) 7 days after bleomycin. We performed gene expression profiling of AMs of broncho-alveolar lavage fluid (BALF) from mice that were intratracheally instilled with saline or bleomycin. Gene expression differences between the two samples could be found using transcriptome profiling (RNA-seq) analysis. RNA profiles were generated by deep sequencing, using Illumina. AMs were stained to confirm the surface expression of CD11c and Siglesc-F. Cells with purity >98% were used for subsequent experiments. We identified several differentially expressed genes in the two groups of cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE252727 | GEO | 2025/12/31

REPOSITORIES: GEO

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