Investigating the role of SNU66 in 5' splice site maintenance

ABSTRACT: CryoEM modeling of the human pre-B complex suggests an interaction between SNU66(H734) and SNRP27(M141) at the base of the ACAGAGA box of U6. We had previously shown in C. elegans that SNRP-27(M141T) mutants alter 5'ss usage. Here we generate new alleles by CRISPR in snu-66 at the corresponding H765 location in C. elegans (H765G and H765L) and demonstrate that they have similar effects as SNRP-27(M141T) mutants on 5'ss selection globally.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE252787 | GEO | 2024/02/20

REPOSITORIES: GEO