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Dual Barcode is Essential for the Specificity of DSB Repair Capture

ABSTRACT: We discovered that PCR-mediated template switching poses a significant challenge in ensemble tagged PCR, particularly in the template sequences with high similarities, which we successfully addressed by introducing a dual barcode. Template switching presents in repair sequencing (repair seq) and severely interfere the interpretation of the association between repair outcomes and the sgRNA in the vicinity. Among the 67 DNA damage response genes, we found that ERCC6L2 was crucial for preventing the DNA end resection.

ORGANISM(S): Escherichia coli Homo sapiens

PROVIDER: GSE253191 | GEO | 2024/04/01

REPOSITORIES: GEO

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