Study of the translation initiation by Selective TCP-seq in the context of eIF1-eIF4G1 inhibitor

ABSTRACT: Start codon recognition by the 48S complex is a critical step in translation. However, understanding the in vivo initiation and its regulation at a global scale is limited. To better understand the mechanism in vivo, we have screened for small molecules that specifically inhibit the function of eIF1-eIF4G1 interaction. We performed Selective-48S footprinting against eIF1 (HA-tagged), eIF2a (HA-tagged), eIF4G1 and eIF3c to answer questions regarding the function of that interaction in the context of the scanning and initiating 48S ribosome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE253652 | GEO | 2024/03/27

REPOSITORIES: GEO