Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Overexpression of DEPDC1B contributes to malignant progression and immune infiltration in lung adenocarcinoma

ABSTRACT: Dysregulated expression of DEPDC1B (DEP domain-containing protein 1B) has been reported to be implicated in various types of malignancies. However, its functional implication as well as the underlying molecular mechanism in lung adenocarcinoma (LUAD) remains elusive. In this study, the role and clinical significance of DEPDC1B in LUAD has been systematically investigated. Our results first showed that DEPDC1B is significantly upregulated in various types of cancers including LUAD, and high DEPDC1B expression is remarkably associated with poor survival in LUAD patients. Functional study demonstrated that DEPDC1B knockdown inhibited cell growth, cell cycle progression, colony formation, cell migration and motility. Furthermore, xenograft nude mouse model experiments showed that DEPDC1B silencing resulted in significant inhibition of tumor growth in vivo. Detailed mechanistic experiments revealed that DEPDC1B knockdown caused down-regulation of various downstream genes including NID1, FN1 and EGFR, and inactivation of multiple critical pathways including ERK and PI3K-AKT pathways.

ORGANISM(S): Homo sapiens

PROVIDER: GSE253772 | GEO | 2025/04/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Nidogen-1, a player in KMT2A rearragned pediatric acute myeloid leukemia

Project description:Despite advances in outcome, one third of children with acute myeloid leukemia (AML) relapse, and less than half will achieve long-term survival. Relapse in AML has shown to be driven in part by the leukemic stem cells (LSC), highlighting the unmet medical need to better characterize and target this therapy resistant cell population. Micro-array profiling of pediatric AML subpopulations (LSC and leukemic myelo-blasts) and their healthy counterparts, revealed Nidogen-1 (NID1) as expressed in both leukemic subpopulations, while absent in the hematopoietic stem cell. Using the TAR-GET dataset including pediatric AML patients (n=1025), NID1 expression showed to correlate with worse event-free survival and KMT2A-rearrangements. Drug response profiling of a NID1 knockdown model demonstrated differential sensitivity to HSP90 inhibition. RNA sequencing and gene set enrichment analysis between NID1high and NID1low phenotypes showed involvement of NID1 in mitochondrial metabolic path-ways known to be enriched in the LSC. Altogether, this study highlights NID1 as a novel oncogene associated with worse EFS and metabolic LSC phenotype in AML. NID1 could serve as a biomarker and aid in further mapping LSCs to establish thera-peutic strategies tackling the high relapse rates in pediatric AML.

2025-04-17 | GSE292050 | GEO

A novel lncRNA, LUADT1 promotes lung adenocarcinoma proliferation by epigenetic suppression of p27

Project description:Long noncoding RNAs (lncRNAs) are known to regulate the development and progression of various cancers, however, few lncRNAs have been well characterized in lung adenocarcinoma (LUAD). Understanding the expression profile of lncRNAs and protein-coding genes is critical to develop new diagnosis and treatment strategies for LUAD and improving the prognosis of diagnosed patients. Five female LUAD patients with no smoking history were selected to profile lncRNA and protein-coding gene expression with microarrays. Paired tumor tissues and adjacent nontumor tissues were collected and confirmed by pathologists.

2015-03-10 | E-GEOD-66654 | biostudies-arrayexpress

Depdc1b

Project description:Rattus norvegicus Depdc1b, DEP domain containing 1B [Source:RGD Symbol;Acc:1305799], is differentially expressed in 27 experiment(s);

| ENSRNOG00000010701 | ExpressionAtlas

Depdc1b

Project description:Rattus norvegicus Depdc1b, DEP domain containing 1B [Source:RGD Symbol;Acc:1305799], is expressed in 4 baseline experiment(s);

| ENSRNOG00000010701 | ExpressionAtlas

KIF2C is a critical regulator for malignant progression of head and neck squamous cell carcinoma

Project description:Head and neck squamous cell carcinoma(HNSCC) is a significant cause of mortality, while the underlying mechanism remains unclear. Recent studies indicate that KIF2C could play a vital role in tumor proliferation and metastasis. Our results demonstrate that KIF2C is highly expressed at both the mRNA and protein levels in HNSCC samples compared to normal samples. Gene ontol-ogy and Kyoto Encyclopedia of Genes and Genomes pathway analyses showed that differentially expressed genes were enriched in processes or pathways related to cell adhesion and cell mitosis in HNSCC. Additionally, a protein-protein interaction network was established, identifying KIF2C as a potential hub gene in HNSCC. Cell phenotype assays showed that KIF2C knockdown reduces cell migration and invasion ability and exhibited cell cycle arrest, high proportion of abnormal cell apoptosis and cell chromosome division mismatches in HNSCC cell line. RNA-seq and RT-PCR assay results revealed that KIF2C knockdown influenced the expression levels of PDGFA, CCNA1, RRM1, CCND2, CAV1, EGFR, SNAI2, KRT5, KRT14, NID1, TP63. TGFBR2, TGFB2, ICAM1, ITGB2, CDH2, CDH5, FN1. And KIF2C knockdown also decreased the phos-phorylation of mTOR, AKT, and ERK. Furthermore, in vivo studies have demonstrated that KIF2C knockdown significantly inhibits tumor proliferation in nude mice. As such, our study highlights the potential therapeutic role of KIF2C for HNSCC treatment.

2024-01-21 | GSE253500 | GEO

DEPDC1B

Project description:Sus scrofa DEPDC1B, DEP domain containing 1B [Source:HGNC Symbol;Acc:HGNC:24902], is expressed in 1 baseline experiment(s);

| ENSSSCG00000035012 | ExpressionAtlas

DEPDC1B

Project description:Macaca mulatta DEPDC1B, DEP domain containing 1B [Source:VGNC Symbol;Acc:VGNC:71705], is expressed in 2 baseline experiment(s);

| ENSMMUG00000018379 | ExpressionAtlas

DEPDC1B

Project description:Homo sapiens DEPDC1B, DEP domain containing 1B [Source:HGNC Symbol;Acc:HGNC:24902], is differentially expressed in 185 experiment(s);

| ENSG00000035499 | ExpressionAtlas

DEPDC1B

Project description:Monodelphis domestica DEPDC1B, DEP domain containing 1B [Source:HGNC Symbol;Acc:HGNC:24902], is expressed in 1 baseline experiment(s);

| ENSMODG00000019582 | ExpressionAtlas

DEPDC1B

Project description:Oryctolagus cuniculus DEPDC1B, DEP domain containing 1B [Source:HGNC Symbol;Acc:HGNC:24902], is expressed in 1 baseline experiment(s);

| ENSOCUG00000016650 | ExpressionAtlas

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data