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Perturb-seq analysis using genes associated with alcohol consumption and alcohol use disorder

ABSTRACT: Although genome-wide association studies (GWAS) have identified loci associated with alcohol consumption and alcohol use disorder (AUD), they do not identify which variants are functional. To approach this, we evaluated the impact of variants in 3’ untranslated regions (3’-UTRs) of genes in loci associated with substance use and neurological disorders using a massively parallel reporter assay (MPRA) in neuroblastoma and microglia cells. We identified genes whose 3’-UTR activities are associated with AUD and alcohol consumption by combining variant effects from MPRA with GWAS results. We examined their effects by evaluating gene expression after CRISPR inhibition of neuronal cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE253842 | GEO | 2026/06/15

REPOSITORIES: GEO

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Massively Parallel Reporter Assay for 3'-UTR Variants Associated with Neurological Disorders in brain tissue cell lines

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