HuR targetome analysis in THP-1 macrophages using HuR FLASH-Seq.

ABSTRACT: FLASH, a novel method, was utilized to identify binding sites of RNA-binding proteins (RBPs) with single-nucleotide resolution (Nucleic Acids Res 2020;48:e15). In order to identify HuR target genes in THP-1 macrophages after circARCN1 regulation, we performed FLASH experiments using the anti-HuR antibody on THP-1 macrophages with circARCN1 knockdown or overexpression. The resulting FLASH products were then subjected to RNA sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE254370 | GEO | 2024/06/01

REPOSITORIES: GEO