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Comparison of the response to bile of Lactobacillus casei BL23 and its derivative strain TC01

ABSTRACT: The response to bile of Lactobacillus casei BL23 and its derivative strain TC01 was investigated. TC01 strain carries a complete deletion of gene LCABL_02080 which encodes a two component signal transduction response regulator.

ORGANISM(S): Lacticaseibacillus casei Lacticaseibacillus paracasei ATCC 334

PROVIDER: GSE25448 | GEO | 2012/10/10

SECONDARY ACCESSION(S): PRJNA133809

REPOSITORIES: GEO

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Comparison of the response to bile of Lactobacillus casei BL23 and its derivative strain TC01

Project description:The response to bile of Lactobacillus casei BL23 and its derivative strain TC01 was investigated. TC01 strain carries a complete deletion of gene LCABL_02080 which encodes a two component signal transduction response regulator. Lactobacillus casei BL23 and TC01 strains were grown in MRS medium at 37C without shaking until O.D. 595 nm. reached 0.5. The cultures were then two fold diluted with prewarmed MRS medium (control) or MRS supplemented with 0.2% bovine bile (treatment) and incubation continued for 45 min. Cells were then harvested and total RNA purified.

2012-10-10 | E-GEOD-25448 | biostudies-arrayexpress

Response to bile of Lactobacillus casei BL23

Project description:The response to bile of Lactobacillus casei BL23 was investigated.

2011-10-20 | GSE33047 | GEO

Response to bile of Lactobacillus casei BL23

Project description:The response to bile of Lactobacillus casei BL23 was investigated. Lactobacillus casei BL23 was grown in MRS medium at 37C without shaking until O.D. 595 nm. reached 0.5. The cultures were then two fold diluted with prewarmed MRS medium (control) or MRS supplemented with 0.2% bovine bile (treatment) and incubation continued for 45 min. Cells were then harvested and total RNA purified.

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Project description:In the present study we characterized the sRNA profile of Lactobacillus casei BL23 using conventional and mDOT-seq approaches. In total 307 potential sRNAs were identified, 28 of which were validated by Northern hybridization. As a major portion of predicted sRNAs were shown to be conserved among the related species of Lactobacillus casei group (LCG), the results obtained in this study could serve as a valuable resource for achieving a better understanding of LCG physiology or even improving their applications in industry and medicine.

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Project description:Lactobacillus casei is remarkably adaptive to diverse habitats. To understand the evolution and adaptation of Lb. casei strains isolated from different environments, the gene content of 22 Lb. casei strains isolated from various habitats (cheeses, n=8; plant materials, n=8; and human sources, n=6) were examined by comparative genome hybridization with an Lb. casei ATCC 334-based microarray. Comparative genome hybridization was performed against an Affymetrix custom microarray designed to include 2,661 (97%) chromosomal and 17 (85%) plasmid CDSs predicted to occur in Lb. casei ATCC 334, as well as all predicted CDSs in the draft Lb. helveticus CNRZ 32 genome. CDSs that were not included in the microarray design were all transposase-encoding genes.

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Project description:Comparison of the response to bile of Lactobacillus casei BL23 and its derivative strain TC01

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Proteomic analysis of the effect of DNA methylation on Lactobacillus casei

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Surface proteome of Lactobacillus casei GCRL163 under heat adaptation

Project description:Cell surface proteins are constatntly impacted by environmental stresses. To understand the imapct of heat adaptation on the Lactobacillus casei GCRL163, trypsin shaving technique was used to extract the surface proteins of the strain and the protein fraction obtained analysed using gel-free proteomics.

2022-03-01 | PXD009468 | Pride

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