RNA-seq analysis of differentially expressed genes between WT and shTRIM21 RKO cells.

ABSTRACT: To investigate the biological function TRIM21 in the colorectal cancer, we established RKO cell lines in which the TRIM21 gene has been knocked down by shRNA. We used a lentiviral shRNA technique to knockdown TRIM21 expression in RKO cells. A scrambled shRNA (shRNA/Control) was used as a control. We then performed RNA-Seq with Cloud-seq Biotech Inc. (Shanghai, China) to analyze gene expression changes in RKO cells. For each sample, three independent biological replicates were performed.

ORGANISM(S): Homo sapiens

PROVIDER: GSE254956 | GEO | 2025/08/22

REPOSITORIES: GEO