EC-8042 reverses the increased SP/KLFs chromatin binding caused by HDACi in H3K27M-DIPG
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ABSTRACT: Diffuse intrinsic pontine gliomas (DIPGs) are lethal pediatric brain tumors with limited therapeutic options. Frequently harboring H3K27M mutations, these tumors are resistant to Histone deacetylase inhibitors (HDACi) in clinical trials for yet unclear reasons. Given this, there is a critical need for exploring the reasons of insufficient clinical manifestations of HDACi and identifying effective combinatorial therapeutic strategy for the treatment of DIPG. To explore the possibility of combining HDACi with EC-8042, an analog of Mithramycin that blocks the DNA binding of SP/KLF factors,We performed CUT&Tag of SP1 in SU-DIPG-XVII cells after treated with DMSO, vorinostat and combination vorinostat with EC-8042. And discovered that EC-8042 reverses the increased SP/KLFs chromatin binding caused by HDACi.
ORGANISM(S): Homo sapiens
PROVIDER: GSE255012 | GEO | 2024/02/08
REPOSITORIES: GEO
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