Dataset Information


PI3K/mTOR specific phospho-antibody microarray to study the effect of rapamycin to the innate immune respsonse

ABSTRACT: To comprehensively analyze the effects of mTORC1 inhibition on GSK3, we employed the use of a PI3K/mTOR-specific phospho-antibody microarray that analyzed the site-specific phosphorylation of over 130 kinases within the PI3K/mTOR pathway. The phosphorylation levels of different kinases in monocytes were measured when stimulated with LPS in the presence or absence of a kind of mTORC1 inhibitor, rapamycin Overall design: More than 130 highly specific and characterized phospho-antibodies for the human mTOR signaling pathway were immobilized and replicated six times on glass slides. The same non-phosphorylated target antibodies were included to allow the determination of the relative level of phosphorylatioin

INSTRUMENT(S): mTOR Signaling Phospho Specific Antibody Microarray

ORGANISM(S): Homo sapiens  

SUBMITTER: huizhi wang  

PROVIDER: GSE25520 | GEO | 2010-11-25



Similar Datasets

2010-11-25 | E-GEOD-25520 | ArrayExpress
2016-07-01 | E-GEOD-79492 | ArrayExpress
2010-06-24 | E-GEOD-13808 | ArrayExpress
| GSE81844 | GEO
| GSE84919 | GEO
2008-12-05 | GSE13808 | GEO
2016-12-12 | PXD003073 | Pride
2017-05-18 | MODEL1705030000 | BioModels
| GSE76766 | GEO
2015-05-07 | BIOMD0000000640 | BioModels