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Dataset Information

52

PI3K/mTOR specific phospho-antibody microarray to study the effect of rapamycin to the innate immune respsonse


ABSTRACT: To comprehensively analyze the effects of mTORC1 inhibition on GSK3, we employed the use of a PI3K/mTOR-specific phospho-antibody microarray that analyzed the site-specific phosphorylation of over 130 kinases within the PI3K/mTOR pathway. The phosphorylation levels of different kinases in monocytes were measured when stimulated with LPS in the presence or absence of a kind of mTORC1 inhibitor, rapamycin Overall design: More than 130 highly specific and characterized phospho-antibodies for the human mTOR signaling pathway were immobilized and replicated six times on glass slides. The same non-phosphorylated target antibodies were included to allow the determination of the relative level of phosphorylatioin

INSTRUMENT(S): mTOR Signaling Phospho Specific Antibody Microarray

ORGANISM(S): Homo sapiens  

SUBMITTER: huizhi wang  

PROVIDER: GSE25520 | GEO | 2010-11-25

SECONDARY ACCESSION(S): PRJNA133777

REPOSITORIES: GEO

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