Transcriptomics,Genomics

Dataset Information

41

Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans


ABSTRACT: Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans. Naïve and activated CD4 T cells, CD8 T cells and B cells were compared for their N-linked glycan structures by MALDI-TOF MS profiling and for expression of glycan transferase genes to assess the biosynthetic basis for any change observed. The major change observed in activated CD4 and CD8 T cells was dramatic reduction of sialylated bi-antennary N-glycans carrying the terminal NeuGc?2-6Gal sequence, and corresponding increase in glycans carrying the Gal?1-3Gal sequence. This change was accounted for by a decrease in the expression of the sialyltransferase ST6Gal, and increase in the expression of the galactosyltransferase ?1-3GalT. Conversely, in B cells no change in terminal sialylation of N-linked glycans was evident, and the expression of the same two glycosyltransferases were increased and decreased, respectively. Keywords = N-linked glycosylation, T cell, B cell, activation, glycosyltransferase, carbohydrate, glycomics, glycan, galactosyltransferase, sialyltransferase Keywords: other

INSTRUMENT(S): GLYCOv1 glycogene array

SUBMITTER: Jennifer A Hammond  

PROVIDER: GSE2554 | GEO | 2005-05-03

SECONDARY ACCESSION(S): PRJNA92163

REPOSITORIES: GEO

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Activation of murine CD4+ and CD8+ T lymphocytes leads to dramatic remodeling of N-linked glycans.

Comelli Elena M EM   Sutton-Smith Mark M   Yan Qi Q   Amado Margarida M   Panico Maria M   Gilmartin Tim T   Whisenant Thomas T   Lanigan Caroline M CM   Head Steven R SR   Goldberg David D   Morris Howard R HR   Dell Anne A   Paulson James C JC  

Journal of immunology (Baltimore, Md. : 1950) 20060801 4


Differentiation and activation of lymphocytes are documented to result in changes in glycosylation associated with biologically important consequences. In this report, we have systematically examined global changes in N-linked glycosylation following activation of murine CD4 T cells, CD8 T cells, and B cells by MALDI-TOF mass spectrometry profiling, and investigated the molecular basis for those changes by assessing alterations in the expression of glycan transferase genes. Surprisingly, the maj  ...[more]

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