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Vastus lateralis muscle biopsy: post-expedition vs. pre-expedition

ABSTRACT: To define the skeletal muscle adaptations induced by exercise performed at high altitude hypoxia, we investigated if the gene expression profile of Vastus lateralis muscle was affected by 5000 m-above-sl-expedition.

ORGANISM(S): Homo sapiens

PROVIDER: GSE25618 | GEO | 2010/11/27

SECONDARY ACCESSION(S): PRJNA133963

REPOSITORIES: GEO

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Vastus lateralis muscle biopsy: post-expedition vs. pre-expedition

Project description:To define the skeletal muscle adaptations induced by exercise performed at high altitude hypoxia, we investigated if the gene expression profile of Vastus lateralis muscle was affected by 5000 m-above-sl-expedition. Two-condition experiment, Vastus lateralis muscle biopsy post-expedition vs Vastus lateralis muscle biopsy pre-expedition. Five volunteers/climbers. Biological replicates: 1 pre-expedition sample, 1 post-expedition sample.

2010-11-27 | E-GEOD-25618 | biostudies-arrayexpress

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Project description:Changes in Gene exporession after 8 weeks of PrimaVie Shilajit Supplementation were measured in vastus lateralis GeneChip® Human Transcriptome Array 2.0 was used to study the gene expression in human vastus lateralis after supplementation of PrimaVie Shilajit and identified distinct classes of up-regulated genes during this process.

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Vastus lateralis muscle of patients with an acute COPD exacerbation

Project description:To further unravel the potential molecular mechanisms involved in the loss of muscle function during an acute exacerbation, a cross-sectional microarray study was designed to compare the gene expression profile of the vastus lateralis muscle in patients with an acute COPD exacerbation and in stable COPD patients. Keywords: cross-sectional patient study A cross-sectional microarray study was designed. The microarray screening was performed on a vastus lateralis biopsy obtained from 4 patients with an acute COPD exacerbation and 5 stable COPD patients. No replicates or dye-swaps were included.

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Gene expression in rhesus monkey vastus lateralis muscle in response to long-term calorie restriction

Project description:The effect of a long-term calorie restricted diet was evaluated in vastus lateralis muscle of rhesus monkeys For biopsy collection, the animals were given buprenorphine (0.01-0.03 mg/kg, IM) in the morning immediately preceding all biopsies and again that afternoon

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Analysis of vastus lateralis muscle from 30 human subjects classified by metabolic flexibility

Project description:To determine the transcriptomic effects on skeletal muscle gene expression between metabolically flexible and inflexible subjects, we assessed vastus lateralis muscle from 15 males and 15 females using a serial analysis of gene expression strategy. Complementary bioinformatics pathway enrichment studies were performed which indicated that differentially expressed genes between genotypes were predominantly related to various processes include carbohydrate metabolism, lipid metabolism, amino acid metabolism, cell proliferation, and apoptosis.

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Project description:Four healthy human volunteers underwent an acute bout of resistance exercise with the right leg at 2 pm. Biopsies were removed from the Vastus Lateralis muscle 6 h (8 pm) and 18 h (8 am) after exericise

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