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Cyclic compressive loading induces a mature meniscal cell phenotype in mesenchymal stem cells with an atelocollagen-based scaffold

ABSTRACT: we investigated how cyclic compressive loading (CCL) could impact MSCs using three-dimensional cultures in atelocollagen-based meniscal substitute (ACMS). We extracted MSCs from the meniscus, synovium, and articular cartilage, cultured them in three-dimensional cultures, and submitted to CCL for seven days. We then compared the transcriptomes of MSCs treated with and without CCL. Our RNA-seq analysis revealed that CCL induced significant transcriptome changes, significantly affecting chondrocyte-related genes, including SOX9, TGFB1, and PRG4 upregulation. CCL-induced transcriptional differentiation from meniscus progenitors toward mature meniscal cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE256243 | GEO | 2024/05/02

REPOSITORIES: GEO

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Effects of dynamic compressive loading on MSC chondrogenesis

Project description:Long-term dynamic compression enhanced the mechanical properties of MSC-seeded constructs only when loading was initiated after 21 days of chondrogenic differentiation. This study examined the molecular differences of chondrogenic MSCs compared to undifferentiated MSCs (TGF-beta vs no TGF-beta) and the effects of dynamic loading on MSC chondrogenesis (loading vs free-swelling). Free-swelling MSC-seeded constructs were cultured for 21 days in chemically defined media. Chondrogenesis was induced with TGF-beta3. Undifferentiated controls were maintained in parallel. After 21 days of chondrogenic differentiation, a subset of constructs were subjected to 21 days of dynamic compressive loading. On days 21 and 42, construct mechanical properties and biochemical content were assessed. Microarray analysis was carried out on day 3, day 21 and day 42 constructs. 6 arrays.

2010-11-01 | E-GEOD-18879 | biostudies-arrayexpress

Differential gene expression of static and intermittent compressive force treated human periodontal ligament cells

Project description:Mechanical force has been shown to regulate periodontal ligament cells (PDLs) behaviors. However, different force types lead to distinct PDLs’ responses. Here, the differential gene expression profiling of PDLs subjected to static and intermittent compressive forces was examined using RNA sequencing technique. Results demonstrated that the static and intermittent compressive force treated PDLs exhibited the differential regulation genes. KEGG pathway enrichment analysis revealed that focal adhesion and transforming growth factor beta signaling pathway were commonly upregulated while calcium signaling pathway was downregulated in both static and intermittent compressive force-treated PDLs. Interestingly, Wnt signaling pathway was upregulated only in the PDLs that subjected to the intermittent compressive force.

2019-07-23 | GSE112122 | GEO

Integrated Analysis of lncRNA and mRNA Expression Profiles Indicates Age-related Changes in Meniscus

Project description:Little has been known about the role of long non-coding RNA (lncRNA) involves in meniscus degradation with aging.In order to investigate the molecular mechanisms of age-related meniscal degeneration and further explore potential biologic therapeutic targets, we performed microarray analysis of meniscus to determine lncRNAs and mRNAs expression profiles in young and aging adults. Then, we carried out bioinformatics analysis of differentially expressed molecules to identify key molecules and functional pathways in the aged meniscus tissues.

2022-04-06 | GSE191157 | GEO

Intracellular free zinc mediates compressive stress-induced mechanotransduction in pancreatic cancer

Project description:In this study, Nihei et al. show that unknown function of the intracellular free zinc in mechanical compressive stress-induced signaling and altered gene expression in pancreatic cancer cells, provide novel perspectives on zinc as a signal transducer and therapeutic target. We explored the roles of compressive stress in pancreatic cancer progression by investigating the changes in gene expression induced by it using microarray.

2022-08-01 | GSE196275 | GEO

Tryptase regulation of joint lubrication and inflammation in osteoarthritis

Project description:Proteoglycan 4 (PRG4) is an extracellular matrix protein that maintains homeostasis through its boundary lubricating and anti-inflammatory properties. Altered expression and function of PRG4 have been associated with joint inflammatory diseases, including osteoarthritis (OA). We found that mast cell tryptase cleaves PRG4 in a dose- and time-dependent manner, which was confirmed by silver stain gel electrophoresis and mass spectrometry. Tryptase-treated PRG4 resulted in a reduction of lubrication. Compared to full-length, processed PRG4 was shown to further activate NF-B expression in cells overexpressing TLR2, -4, and -5. In the destabilization of the medial meniscus (DMM) model of OA in rat, tryptase and PRG4 colocalized at the site of injury in knee cartilage and were associated with disease severity. Primary synovial fibroblasts from OA patients or healthy subjects treated with tryptase and/or PRG4, and synovial cells from OA patients, were subjected to a quantitative shotgun proteomics and proteome changes were characterized, further supporting the role of NF-B activation. We identified tryptase as a modulator of joint lubrication via the processing of PRG4 in OA.

2023-03-04 | PXD037040 | Pride

Differential gene expression of intermittent compressive force treated mouse gingival fibrpblast derived induced pluripiotent stem cells

Project description:Mechanical force regulates various biological responses in many cell types. Here, the retinoic acid treated mouse gingival fibroblast derived induced pluripotent stem cells were treated with intermittent compressive for 24 h and gene expression profiling was evaluated using RNA sequencing technique. Results indicated that intermittent compressive stress regulated various pathways in these cells including p53 signaling and other metabolic pathways.

2021-12-31 | GSE118952 | GEO

The long non-coding RNA landscape of periodontal ligament stem cells subjected to compressive force

Project description:To explore the lncRNA landscape of periodontal ligament stem cells (PDLSCs) subjected to compressive force.

2021-07-09 | GSE116787 | GEO

RNA sequencing of human meniscal fibrochondrocytes treated with 17beta-estradiol

Project description:Meniscal tears are the most prevalent intra-articular knee injury and pose significant risk in the development of knee osteoarthritis, a progressive disease that affects around 250 million people worldwide. Meniscus tissue, especially the avascular inner third, heals poorly and the only treatment option is surgery. Epidemiological data and the vital role of estrogen in tissue repair and homeostasis indicate that estrogen could be fundamental for knee joint health and could be useful for tissue regeneration. However, the specific cell response to estrogen and the differences in response of cells from different donors is unknown. In this study, RNA sequencing was used to characterize the mRNA transcriptome of human meniscal cells from male (47-year-old) and female (16-, 17-, and 47-year-old) donors treated with 17beta-estradiol. The goal is to provide data valuable to the design and implementation of estrogen replacement therapies for meniscal health and osteoarthritis reduction.

2022-09-16 | GSE199087 | GEO

Immunomodulatory Effects of Massage on Non-Perturbed Skeletal Muscle in Rats

Project description:Microarray was used as a screening tool to determine changes in gene transcription with cyclic compressive loading (CCL) (massage mimetic) applied at varying magnitudes in non-perturbed skeletal muscle. 24 male Wistar Rats were randomly assigned to one of three groups based on magnitude of load: Control: 0N; Low Load: 1.4N, and Moderate Load 4.5N. Animals were anesthetized using isofluorane, postioned lateral-recumbant with one limb secured to a platform with the Tibialis Anterior (TA) muscle facing superiorly. Using a custom CCL device, load was applied along the length of the TA cycled at 15mm proximally and distally at a frequency of 0.5Hz. Each animal received a 30-min bout of CCL once a day for four consecutive days. Rats were euthanized 24 hour following the fourth treatment. TA muscles were harvested and preserved cryogenically. Muscle tissue was homogenized, and RNA was isolated using ToTALLY RNA kit. Microarray was performed using Affymetrix GeneChip system.

2013-09-01 | E-MEXP-3957 | biostudies-arrayexpress

proteomic profiling of human meniscus

Project description:There is a need to clarify the role of the meniscus in knee OA which may be aided by the identification of biomarkers that reflect the molecular events involved in the tissue degradation. This is of particular importance in the early stages of the degenerative processes, a crucial phase when it might still be possible to interfere with disease progression before tearing and critical loss of meniscal function occurs. One way to achieve this goal is exploratory proteomics by mass spectrometry (MS), which has the potential to analyse the proteome of complex samples. Thus, our aim was to gain new knowledge about the molecular processes within the human meniscus by comparing the proteomic profiles of both the lateral and medial meniscus from healthy controls, subjects with mild signs of joint degeneration, and patients with end-stage knee OA, using a non-targeted MS approach operating in data-independent acquisition (DIA) mode. For the research purpose six groups were established: medial and lateral menisci from control donors (n=12), medial and lateral menisci from donors with mild signs of joint degeneration (n=13) and medial and lateral menisci from end-stage knee OA patients (n=12).

2024-04-03 | PXD042122 | Pride

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