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Screening of NFIL3 target genes (Affymetrix HTA 2.0 data set)

ABSTRACT: To identify the target gene of NFIL3, we transfected K562 cells with siNFIL3 or siControl, respectively. In order to better understand the function of NFIL3 gene, mRNA expression profiles were analyzed by using Affymetrix HTA2.0 Microarray in K562 cells transfected with siNFIL3 or siControl under hypoxia

ORGANISM(S): Homo sapiens

PROVIDER: GSE256294 | GEO | 2024/02/26

REPOSITORIES: GEO

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Differential requirement for Nfil3 during NK cell development

Project description:Nfil3 has multiple functions during conventional NK cell and thymic NK cell development. Nfil3 controls NK cell development from the earliest committed NK cell progenitor stage. Nfil3-/- prepro NK cells retained T-cell potential due to the failure to repress T cell specific genes. At later stages of NK cell development, Nfil3 regulates the formation of mature NK cells by promoting directly or indirectly the expression of transcription factor Eomes. We also demonstrate that Nfil3 is important for thymic NK cell development whereas Trail+ immature NK cells generated in the bone marrow of neonates and in the adult liver develop independent of Nfil3 function. We used microarrays to analyse the changes in gene expression due to the presence or absence of Nfil3, both in prepro NK cells and ALP cells (all lymphoid progenitors)

2013-10-08 | GSE45955 | GEO

Differential requirement for Nfil3 during NK cell development

2013-10-08 | E-GEOD-45955 | biostudies-arrayexpress

Differential requirement for Nfil3 during NK cell development

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2014-05-07 | E-GEOD-54291 | biostudies-arrayexpress

Differential requirement for Nfil3 during NK cell development

2014-05-07 | GSE54291 | GEO

HEK293 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from HEK293 cells that were infected with control or NFIL3 shRNA Two condition experiment: control (scr) shRNA (six replicates) and NFIL3 shRNA (four replicates)

2012-09-02 | E-GEOD-31426 | biostudies-arrayexpress

BT549 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from BT549 cells that were infected with control or NFIL3 shRNA Two condition experiment: control (scr) shRNA (two replicates) and NFIL3 shRNA (two replicates)

2012-09-02 | E-GEOD-31425 | biostudies-arrayexpress

Diminishment of NFIL3 by shRNA in HEK293 and BT549 cells

Project description:This SuperSeries is composed of the following subset Series: GSE31425: BT549 cells with control or NFIL3 shRNA treatment GSE31426: HEK293 cells with control or NFIL3 shRNA treatment Refer to individual Series

2012-09-02 | E-GEOD-31427 | biostudies-arrayexpress

HEK293 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from HEK293 cells that were infected with control or NFIL3 shRNA

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Nfil3, a target of the NACA transcriptional coregulator, affects osteoblast and osteocyte gene expression differentially

Project description:Using ChIP-Seq, we show that Nfil3 is a transcriptional target of the PTH-NACA axis in osteoblasts. We also show that Nfil3 transcription is, by part, regulated by NACA and CREB. Finally, we show that Nfil3 has a distinct role in osteoblasts vs osteocytes

2020-03-17 | GSE147070 | GEO

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