Transcriptomics

Dataset Information

47

Expression Profiling of Erythroid Progenitors After MYB shRNA Knockdown


ABSTRACT: MYB plays a critical role as a regulator of erythropoieisis. We have shown that MYB silences epsilon and gamma-globin expression in erythroid progenitors. We here examine erythroid cells at the basophilic erythroblast stage of differentiation with MYB shRNA or control lentiviral transduction prior to differentiation. Overall design: We have cultured CD34 cells and transduced the cells with lentiviruses harboring shRNAs targeting MYB or controls and then allowed the cells to differentiate down the erythroid lineage. At the basophilic erythroblast stage of differentiation, the cells were harvested and total RNA was extracted. This was used to hybridize to Affymetrix expression arrays using the HG-U133 Plus 2.0 platform to ascertain expression differences that occur with the knockdown of MYB.

REANALYSED by: GSE36988E-GEOD-36994

INSTRUMENT(S): Affymetrix GeneChip Human Genome U133 Plus 2.0 Array [CDF: HGU133Plus2_Hs_ENTREZG v11.0.1]

ORGANISM(S): Homo sapiens  

SUBMITTER: Vijay G. Sankaran  

PROVIDER: GSE25678 | GEO | 2011-01-03

SECONDARY ACCESSION(S): PRJNA135825

REPOSITORIES: GEO

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