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Rnai tor time course - The signalization way TOR/PTEN in Arabidopsis

ABSTRACT: gnp_blan06_torpten - rnai tor time course - The impact of the TOR pathway on growth and stress responses. Modification of translational and transcriptional profiles in Tor-inducible RNAi mutants and identification of TOR targets.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE25701 | GEO | 2011/01/07

SECONDARY ACCESSION(S): PRJNA134113

REPOSITORIES: GEO

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rnai tor time course - The signalization way TOR/PTEN in Arabidopsis

Project description:gnp_blan06_torpten - rnai tor time course - The impact of the TOR pathway on growth and stress responses. Modification of translational and transcriptional profiles in Tor-inducible RNAi mutants and identification of TOR targets. 28 dye-swaps - normal vs. rnai mutant comparison.

2011-01-07 | E-GEOD-25701 | biostudies-arrayexpress

dark hypocotyls tor rnai-Dark hypocotyls TOR RNAi

Project description:fapesp-bra-inra-10-01_bioen_hypocotyl - dark hypocotyls tor rnai - Transcriptional comparison between 2 TOR RNAi mutants versus GUS control. - Sowing after 24h imbibition at 4°C in the dark, on MS1/5, no sucrose, 10 mM ethanol, 8 g/l agar, vertical growth with 3h light, 6 days growth in the dark (20°C), hypocotyls were harvested under green light ; cotyledons and root were removed.

2012-10-10 | GSE41404 | GEO

dark hypocotyls tor rnai-Dark hypocotyls TOR RNAi

Project description:fapesp-bra-inra-10-01_bioen_hypocotyl - dark hypocotyls tor rnai - Transcriptional comparison between 2 TOR RNAi mutants versus GUS control. - Sowing after 24h imbibition at 4M-BM-0C in the dark, on MS1/5, no sucrose, 10 mM ethanol, 8 g/l agar, vertical growth with 3h light, 6 days growth in the dark (20M-BM-0C), hypocotyls were harvested under green light ; cotyledons and root were removed. 4 dye-swap - normal vs rnai mutant comparaison

2012-10-10 | E-GEOD-41404 | biostudies-arrayexpress

lst8 - The signalisation way TOR/PTEN in Arabidopsis

Project description:gnp_blan06_torpten - lst8 - The impact of the TOR pathway on growth and stress responses. Comparison between the lst8 mutant vs. WT in short day and in long day conditions.

2010-12-08 | GSE25731 | GEO

lst8 - The signalisation way TOR/PTEN in Arabidopsis

Project description:gnp_blan06_torpten - lst8 - The impact of the TOR pathway on growth and stress responses. Comparison between the lst8 mutant vs. WT in short day and in long day conditions. 8 dye-swaps - gene knock out.

2010-12-08 | E-GEOD-25731 | biostudies-arrayexpress

Identification of glucose-TOR signaling early target genes in Arabidopsis seedling autotrophic transition stage

Project description:The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR. Arabidopsis WT and estradiol inducible RNAi-tor seedlings were germinated in low light condition for 3 days to deplete seed nutrient supply. Fifteen mM glucose was adding back to reactivate the quiescent seedlings and the samples were harvested for RNA isolation. Transcript profiles were analyzed and compared between with and without glucose treatment to identify early glucose-TOR target genes. Each experimental condition was repeated three times and total 12 samples were generated.

2013-04-05 | E-GEOD-40245 | biostudies-arrayexpress

Transcription profiling by high throughput sequencing of Arabidopsis estradiol-inducible tor-es mutant

Project description:To validate that the transcriptional program rapidly activated by PP242, WYE354, KU63794 and Torin2 was specifically associated with the complete inhibition of TOR kinase, we performed genome-wide transcript profiling using the estradiol-inducible tor-es mutant after reaching complete TOR protein silencing in Arabidopsis seedlings grown in the full-nutrient medium. The estradiol inducible tor-es mutant was treated without/with estradiol for 72 hours to complete inhibition of TOR kinase. And RNA-seq was performed for comparison the transcriptomes changes (http://gbnci.abcc.ncifcrf.gov/geo/gse_view.php?ID=58096).

2015-06-08 | E-MTAB-4132 | biostudies-arrayexpress

Overexpression of pten2a: The signaling pathway of TOR/PTEN in Arabidopsis

Project description:gnp_blan06_torpten - overexpression of pten2a - The impact of the TOR pathway on growth and stress responses. - Comparaison control versus overexpressor. The overexpressor has a male phenotype of sterility.

2010-11-30 | GSE24902 | GEO

Human and Mouse Cells Time of Replication (ToR)

Project description:This SuperSeries is composed of the following subset Series: GSE17229: Mouse Cells Time of Replication (ToR) GSE17235: Human Cells Time of Replication (ToR) Refer to individual Series

2010-05-25 | E-GEOD-17236 | biostudies-arrayexpress

Comparative proteomic analysis to study the involvement of the Tor pathway in the human-pathogenic fungus Aspergillus fumigatus

Project description:The Tor kinase is one of the major regulatory nodes in a eukaryotic cell and until now, little is known about this kinase in filamentous fungi. Here, we analysed the Tor kinase in Aspergillus fumigatus, which is the most important airborne fungal pathogen of humans and the main causative agent of invasive aspergillosis. Because deletion of the single tor gene of A. fumigatus was apparently lethal, we generated a conditional lethal tor mutant. This was achieved by fusing the tor gene with the inducible xylose promoter and replacing the endogenous tor gene by the inducible xylp-tor gene cassette. The generated transgenic strain opened up the possibility to activate or silence the tor gene under controlled conditions. Since we did not observe any effect of rapamycin on Tor of A. fumigatus, the generation of the inducible Tor gene was even more valuable for an in-depth analysis of the Tor kinase. By LC-MS/MS analyses of mycelial proteins we identified the regulatory network of Tor. Tor controls a variety of genes and proteins involved in nutrient sensing, stress response, cell cycle progression, protein biosynthesis and degradation. Tor is a major kinase responsible for protein import into the mitochondria, and consequently responsible for the correct function of most processes occurring into these organelles, e.g. respiration and ornithine metabolism. Regulation of iron acquisition by Tor was found to be independent of the HapX transcription factor.

2022-02-28 | PXD001601 | Pride

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