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[E-MTAB-345] ChIP-Seq of human MCF-7 cells with anti-ERalpha following estrogen treatment

ABSTRACT: We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. (Note: Raw data files not provided by submitter) Publication Title: Global analysis of ERbeta binding to human breast cancer cell genome Publication Author List: Grober OMV, Mutarelli M, Paris O, Giurato G, De Filippo MR, Cicatiello L, Ravo M, Ferraro L, Nassa G, Tarallo R, Papa MF, Benes V, Alessandro Weisz. ArrayExpress Release Date: 2010-10-05 Person Roles: submitter Person Last Name: Weisz Person First Name: Alessandro Person Mid Initials: Person Email: alessandro.weisz@unina2.it Person Phone: Person Address: Person Affiliation: Second University of Naples

ORGANISM(S): Homo sapiens

PROVIDER: GSE25769 | GEO | 2011/02/05

SECONDARY ACCESSION(S): PRJNA134073

REPOSITORIES: GEO

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[E-MTAB-131] Estrogen Receptor alpha estrogen-responsive binding sites in MCF-7

Project description:We performed chromatin immunoprecipitation (ChIP) with two polyclonal anti-ERalpha antibodies directed against the N- and C-terminus of the protein, with or without estrogen treatment for 45 minutes of MCF-7 cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. ArrayExpress Release Date: 2009-07-27 Publication Title: Estrogen receptor alpha controls a gene network in luminal-like breast cancer cells comprising multiple transcription factors and microRNAs. Publication Author List: Cicatiello L, Mutarelli M, Grober OM, Paris O, Ferraro L, Ravo M, Tarallo R, Luo S, Schroth GP, Seifert M, Zinser C, Chiusano ML, Traini A, De Bortoli M, Weisz A. Person Roles: submitter Person Last Name: Mutarelli Person First Name: Margherita Person Mid Initials: Person Email: mutarelli@tigem.it Person Phone: Person Address: Person Affiliation: Person Roles: investigator Person Last Name: Weisz Person First Name: Alessandro Person Mid Initials: Person Email: alessandro.weisz@unina2.it Person Phone: Person Address: Person Affiliation:

2011-02-05 | GSE25684 | GEO

[E-MTAB-607] Transcription profiling by high throughput sequencing of the planarian Schmidtea mediterranea during head regeneration

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2012-01-20 | GSE30996 | GEO

[E-MTAB-214] ChiP-seq profiling of Drosophila melanogaster salivary glands to identify targets for NSL1 and MCRS2.

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2010-12-04 | GSE25709 | GEO

[E-MTAB-223] ChIP-seq for FOXA1, ER and CTCF in breast cancer cell lines

Project description:Growth cells and map of ER, FoxA1 and CTCF binding at whole genome level. ArrayExpress Release Date: 2010-10-29 Person Roles: submitter Person Last Name: Hurtado Person First Name: Antoni Person Email: toni.hurtado@cancer.org.uk Person Affiliation: Uppsala University

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[E-MTAB-197] CEU_RNASEQ60, Transcription profiling by high throughput sequencing of 60 HapMap CEU population individuals

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2010-12-04 | GSE25030 | GEO

[E-MTAB-75] Cryptic unstable transcripts in yeast

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[E-MTAB-610] Tag profiling of genes from two Pachycladon species

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2011-07-14 | GSE30618 | GEO

[E-MTAB-445] Transcription profiling by high throughput sequencing of blackface sheep infected with the gastrointestinal helminth Teladorsagia circumcincta to analyse resistance to infection

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2011-07-14 | GSE30606 | GEO

[E-TABM-828] ChIP-Seq in human MCF7 and HEPG2 cells

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[E-MTAB-706] Quantitative RNA-seq analysis of the transcriptome of Campylobacter jejuni

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2011-07-14 | GSE30621 | GEO

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