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Affy_infection_medicago-Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the Mtnf-ya1-1 mutant

ABSTRACT: affy_infection_medicago - affy_infection_medicago - MtNF-YA1 is a transcription factor involved in the regulation of nitrogen fixing nodule organogenesis. Mtnf-ya1-1 mutant has been shown to be more resistant to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare both WT and mutant plant responses, 1 and 6 days post inoculation (dpi) between WT and the Mtnf-ya1-1 mutant, in order to identify MtNF-YA1-dependent gene networks during infection. Data about non inoculated A17 control plants and infected A17 at 1 dpi have been already obtained in another experiment in which the conditions of in vitro culture of M. truncatula plants and ofA.euteiches(Ae) inoculation were exactly the same than the ones used in these experiments.-Fifteen-day-old A17 or Mtnf-ya1-1 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with a A. euteiches zoospores (105/ml) and harvested one or six days after inoculation. Three independent repeats were performed.

ORGANISM(S): Medicago sativa Medicago truncatula Sinorhizobium meliloti

PROVIDER: GSE26046 | GEO | 2011/11/01

SECONDARY ACCESSION(S): PRJNA135543

REPOSITORIES: GEO

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affy_infection_medicago-Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the hap2a-1 mutant

Project description:affy_infection_medicago - affy_infection_medicago - HAP2a is a transcription factor involved in the regulation of nitrogen fixing nodule organogenesis. hap2a-1 mutant has been shown to be more resistant to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare both WT and mutant plant responses, 1 and 6 days post inoculation (dpi) between WT and the hap2a-1 mutant, in order to identify HAP2a-dependent gene networks during infection. Data about non inoculated A17 control plants and infected A17 at 1 dpi have been already obtained in another experiment in which the conditions of in vitro culture of M. truncatula plants and ofA.euteiches(Ae) inoculation were exactly the same than the ones used in these experiments.-Fifteen-day-old A17 or hap2a-1 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with a A. euteiches zoospores (105/ml) and harvested one or six days after inoculation. Three independent repeats were performed. 12 arrays - Medicago; normal vs disease comparison,normal vs ems mutant comparison,time course

2011-11-01 | E-GEOD-26046 | biostudies-arrayexpress

Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) and NFP allelic mutants

Project description:affy_aphanomyces_medicago - NFP is one of the putative Nod factor receptor and plays therefore a key role in the establishment of symbiosis. nfp allelic mutants are more susceptible to Aphanomyces euteiches (Ae), a root pathogen, than the A17 wild type (WT) line. In this study we want to compare the early plant responses, 1 day post inoculation (dpi) between WT and the nfp2 mutant, in order to identify NFP-dependent gene networks during infection.-Fifteen-day-old A17 or nfp-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with a A. euteiches zoospores (105/ml) and harvested one day after inoculation. Three independent repeats were performed. Keywords: normal vs ems mutant comparison,near isogenic lines (hif)

2011-09-02 | GSE20587 | GEO

Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) and NFP allelic mutants

2011-09-01 | E-GEOD-20587 | biostudies-arrayexpress

affy_med_2011_10- Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the lyr3-1 and lyr3-2 mutants

Project description:affy_med_2011_10 - affy_med_2011_10 - LYR3 encodes a lysin motif-receptor-like kinase (LysM-RLK) that consists of an extracellular ligand-binding domain composed of three LysM domains, a single transmembrane domain and a cytoplasmic serine/threonine kinase domain. A reverse genetic approach was undertaken to study the role of this gene in the pathosystem Medicago truncatula – Aphanomyces euteiches (Ae). Results suggest that lyr3-1 and lyr3-2 mutants are more susceptible to the root pathogen Aphanomyces euteiches than the A17 wild type (WT) line. In this study, we want to compare the early plant responses, 3 days post inoculation (dpi), between WT and the lyr3-1 and lyr3-2 mutants, in order to identify LYR3-dependent gene networks during infection.-Fourteen days old A17, lyr3-1 or lyr3-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with A. euteiches zoospores (105/ml) and harvested three days after inoculation. Three independent repeats were performed.

2014-02-28 | GSE43715 | GEO

affy_med_2011_10- Comparison of plant responses upon Aphanomyces euteiches infection in wild type (A17) line and in the lyr3-1 and lyr3-2 mutants

Project description:affy_med_2011_10 - affy_med_2011_10 - LYR3 encodes a lysin motif-receptor-like kinase (LysM-RLK) that consists of an extracellular ligand-binding domain composed of three LysM domains, a single transmembrane domain and a cytoplasmic serine/threonine kinase domain. A reverse genetic approach was undertaken to study the role of this gene in the pathosystem Medicago truncatula M-bM-^@M-^S Aphanomyces euteiches (Ae). Results suggest that lyr3-1 and lyr3-2 mutants are more susceptible to the root pathogen Aphanomyces euteiches than the A17 wild type (WT) line. In this study, we want to compare the early plant responses, 3 days post inoculation (dpi), between WT and the lyr3-1 and lyr3-2 mutants, in order to identify LYR3-dependent gene networks during infection.-Fourteen days old A17, lyr3-1 or lyr3-2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = controls) with A. euteiches zoospores (105/ml) and harvested three days after inoculation. Three independent repeats were performed. 18 arrays - Medicago; normal vs mutant comparison,near isogenic lines (hif)

2014-02-28 | E-GEOD-43715 | biostudies-arrayexpress

Molecular mechanisms of partial resistance to Aphanomyces euteiches in Medicago truncatula (affy_molecularmechanism_medicago)

Project description:affy_molecularmechanism_medicago - affy_molecularmechanism_medicago - -Aphanomyces euteiches (Ae), a soilborne oomycete, is the causal agent of pea root rot, a major disease on various cultivated legume species. To gain insight into the mechanism underlying partial resistance to this pathogen, a pathosystem has been developed using the model legume Medicago truncatula (Mt). Two Mt accessions A17 and F83005.5 have been shown to display differential responses upon infection with Ae (partially resistance and susceptibility, respectively). Genetic analyses using a RIL population derived from a cross between those two lines lead to the identification of, prAe1, a major QTL involved in the observed partial resistance. Near isogenic lines (NIL) NR2 and NS2 whose genomes are identical except at the prAe1 locus (resistant alleles for NR2, susceptible alleles for NS2) have been generated, and the same differential response toward Ae has been found. The aim of this experiment is to analyse and compare NR2 and NS2 transcriptomes at 1 and 6 days after Ae inoculation to identify gene networks that are regulated in a prAe1 dependent manner.--Fifteen-day-old NR2 or NS2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = 0dpi) with a A. euteiches zoospores (75000zsp/ml) and harvested one or six days after inoculation. Three independent repeats were performed.

2014-02-28 | GSE31198 | GEO

Molecular mechanisms of partial resistance to Aphanomyces euteiches in Medicago truncatula (affy_molecularmechanism_medicago)

Project description:affy_molecularmechanism_medicago - affy_molecularmechanism_medicago - -Aphanomyces euteiches (Ae), a soilborne oomycete, is the causal agent of pea root rot, a major disease on various cultivated legume species. To gain insight into the mechanism underlying partial resistance to this pathogen, a pathosystem has been developed using the model legume Medicago truncatula (Mt). Two Mt accessions A17 and F83005.5 have been shown to display differential responses upon infection with Ae (partially resistance and susceptibility, respectively). Genetic analyses using a RIL population derived from a cross between those two lines lead to the identification of, prAe1, a major QTL involved in the observed partial resistance. Near isogenic lines (NIL) 5B2 and 5D2 whose genomes are identical except at the prAe1 locus (resistant alleles for 5B2, susceptible alleles for 5D2) have been generated, and the same differential response toward Ae has been found. The aim of this experiment is to analyse and compare 5B2 and 5D2 transcriptomes at 1 and 6 days after Ae inoculation to identify gene networks that are regulated in a prAe1 dependent manner.--Fifteen-day-old 5B2 or 5D2 plants were grown in vitro on M medium. The entire roots were then inoculated (or not = 0dpi) with a A. euteiches zoospores (75000zsp/ml) and harvested one or six days after inoculation. Three independent repeats were performed. 18 arrays - Medicago; resistance vs susceptibility comparison,time course,treated vs untreated comparison

2014-02-28 | E-GEOD-31198 | biostudies-arrayexpress

Comparison of plant responses upon P. palmivora infection in wild type (A17) line and in the api mutant

Project description:api mutant has been shown to be more resistant to P. palmivora than the A17 wild type (WT) line. In this study we want to compare both WT and mutant plant responses to the pathogen as well as their respective control transciptomes in order to identify api-dependent gene networks. Following germination, A17 or api plants grown in vitro on Agar medium were inoculated (or not = controls) with a P. palmivora zoospores (10^5/ml) and harvested 16 hours after inoculation. Three independent repeats were performed.

2016-12-01 | GSE65903 | GEO

affy_infection_medicago - Comparison of plant responses upon P. palmivora infection in wild type (A17) line and in the api mutant

Project description:affy_infection_medicago - api mutant has been shown to be more resistant to P. palmivora than the A17 wild type (WT) line. In this study, we want to compare both WT and mutant plant responses to the pathogen as well as their respective control transcriptomes in order to identify api-dependent gene networks. Following germination, A17 or api plants grown in vitro on Agar medium were inoculated (or not = controls) with a P. palmivora zoospores (10^5/ml) and harvested 16 hours after inoculation. Three independent repeats were performed.

2018-08-27 | GSE119053 | GEO

affy-agro-bi-medicago-AGRO-BI-Toulouse

Project description:affy_agro-bi_medicago - Identification of genes from the model legume Medicago truncatula whose expression is affected by the plant nitrogen status, with or without inoculation with the symbiotic bacteria Sinorhizobium meliloti. - Comparison of the supernodulant, nitrogen-insensitive, sunn-2 mutant with the A17 wild type genotype.-The plant root systems of plants were split into two parts, each one being installed in a separate compartment. For the “S” treatment, one part was supplied with 10 mM NH4NO3 while the other part was supplied with a nitrogen-free nutrient solution. For the “L” treatment, one part was supplied 0.5mM NO3- and the other part was supplied with the nitrogen free solution. Eight biological materials (designated AL, AS, SL, SS, IL, IS, NIL, NIS), with three biological repeats for each, were collected and analyzed. The effects of the S and L conditions were investigated on wild-type A17 (AL vs. AS) and sunn-2 mutant plants (SL vs. SS); one set of A17 plants was inoculated with Sinorhizobium meliloti (IL vs. IS), harvested at four days post inoculation and compared to non-inoculated plants (NIL vs. NIS). Keywords: growth in nitrogen-sufficient (s) vs. nitrogen-limited (l) conditions

2010-10-28 | GSE17253 | GEO

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