Serotonin influence melanocyte lineage specification by affecting Wnt signaling

ABSTRACT: Melanocytes are derived from the neural crest during embryonic development and produce melanin that protect against ultraviolet light damage to the skin and DNA in thermostats, and protects against light and stores heat in metameric animals. Serotonin/5-HT is the important neurotransmitter regulating various physiological processes in the vertebrates. Meanwhile, it has been reported to regulate the cell behavior during early development in a manner similar to growth factors. Previous studies have verified that serotonin regulates melanin synthesis and induces melanoblast specification. However, the underlying mechanisms remain poorly understood. We explore the role of serotonin signaling in zebrafish neural crest cells (NCCs) differentiation through exogenous serotonin treatment. The results suggest that surrounding serotonin is involved in regulating the balance of NCCs differentiated direction into melanocytes or glial cells. We used flow sorting to obtain sox10+ neural crest cells and found that exogenous serotonin regulates melanocyte lineage specialization through Wnt signaling by RNAseq, which indicates the potential application of serotonin signaling manipulation in the therapy of depigmentation cutaneous diseases.

ORGANISM(S): Danio rerio

PROVIDER: GSE260503 | GEO | 2025/07/01

REPOSITORIES: GEO