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Histone H4 Lysine 20 Methylation Marks Genes Dynamically Regulated During Erythroid Maturation (CUT&Tag)

ABSTRACT: We profiled H4K20 mono- and tri- methylation during the well-characterized erythroid differentiation of human hematopoietic stem and progenitor cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE260721 | GEO | 2025/07/30

REPOSITORIES: GEO

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Histone H4 Lysine 20 Methylation Marks Genes Dynamically Regulated During Erythroid Maturation (CUT&RUN)

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Histone H4 Lysine 20 Methylation Marks Genes Dynamically Regulated During Erythroid Maturation (CUT&Tag)

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Histone H4 lysine 20 mono-methylation directly facilitates chromatin ‘openness’ and promotes transcription of housekeeping genes (ChIP-seq)

Project description:To study the role of Histone H4 lysine 20 mono- and tri-methylation (H4K20me1, H4K20me3) in relationship to chromatin accessibility and gene expression, we report the application of high-throughput profiling of these marks in Human Osteosarcoma U2OS and Mouse Fibroblast (MEF) cells with Set8 knockdown or control siRNA treatment. We find that H4K20me1 contributes to the open chromatin structure at active genes.

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Project description:To study the role of Histone H4 lysine 20 mono- and tri-methylation (H4K20me1, H4K20me3) in relationship to chromatin accessibility and gene expression, we report the application of high-throughput profiling of these marks in synchronized and asynchronous Human Osteosarcoma U2OS and asynchronous Mouse Fibroblast (MEF) cells with Set8 knockdown or control siRNA treatment. We find that H4K20me1 contributes to the open chromatin structure at active genes.

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