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FaiR regulates the development of Penicillium oxalicum fungi and the expression of cellulolytic genes

ABSTRACT: The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT) and FaiR deletion strain (DfaiR) under conditions of 1% bran + 1% microcrystalline cellulose as carbon source. The results of correlation analysis between each sample showed that the gene expression level of DfaiR was significantly different from that of the WT strain. The deletion of DfaiR significantly down-regulates the expression levels of sporulation process genes. And the absence of DfaiR can broadly up-regulate the expression level of cellulase-encoding genes, indicating that DfaiR can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes.

ORGANISM(S): Penicillium oxalicum

PROVIDER: GSE261111 | GEO | 2024/03/13

REPOSITORIES: GEO

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The F-box protein Fbx23 regulates the development of Penicillium oxalicum fungi and the expression of cellulolytic genes

Project description:The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), Fbx23 deletion strain (Δfbx23), and Fbx23 ecover strain (Refbx23) under conditions of 2% glucose or 1% bran + 1% microcrystalline cellulose as carbon source. The results of correlation analysis between each sample showed that the gene expression level of Δfbx23 was significantly different from that of the WT strain. The deletion of Fbx23 significantly down-regulates the expression levels of sporulation process genes. And the absence of Fbx23 can broadly up-regulate the expression level of cellulase-encoding genes, indicating that Fbx23 can cause a decrease in cellulase synthesis by affecting the expression of cellulase-encoding genes.

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Project description:The goals of this study are to compare different gene expressions for Penicillium oxalicum wild type strain (WT), and Podot1 knockout strain (ΔPodot1) in different carbon sources. The deletion of Podot1 downregulated genes involved in the septin complex, extracellular region, and interspecies interaction between organisms when strains were cultivated with 2% glucose as carbon sources, and downregulated genes involved in cellulase activity, cellulose binding, glucosidase activity, and polysaccharide catabolic process when strains were cultivated with 1% microcrystalline cellulose and 1% wheat bran as carbon sources. We find the extracellular region was downregulated under both different carbon sources in ΔPodot1. This study provides the information that PoDot1 function are required in mycelial development and hydrolase activity of P. oxalicum.

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Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and its sporogenesis related genes deletion strains

Project description:RNA-seq was used to compare differential gene expressions for Penicillium oxalicum wild type strain (M12) and sporognesis related genes knock out strains.The goals of this study are to construct the sporogenesis regulation pathway of Penicillium oxalicum. Examination of differential gene expressions by digital gene expression tag profiling in Penicillium oxalicum wild type strain and nine mutant strains (flbA knoutout strain, flbB knoutout strain, flbC knoutout strain, flbD knoutout strain, flbE knoutout strain,wetA knoutout strain, abaA knoutout strain,stuA knoutout strain, swi6 knoutout strain)

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Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and its sporogenesis related genes deletion strains

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Transcriptomic analysis of cellulolytic fungus Penicillium oxalicum and transcription factor mutant strains in response to different carbon sources

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