Genome-wide analysis of transfection of miR-141 or miR-200c in murine fibroblasts subjected to acute oxidative stress
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ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE26113 | GEO | 2011/11/01
SECONDARY ACCESSION(S): PRJNA135195
REPOSITORIES: GEO
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Project description:Analysis of abnormal cellular response to stress after overexpression of stress-induced microRNAs. The hypothesis tested in the present study was that miR-141 or miR-200c may influence transcription of stress-pathways related genes Total RNA obtained from fibroblasts transfected for 48h with Control, miR-141 or miR-200c. 2h hours before lysis, fibroblasts were stimulated with 400 uM H2O2.
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Project description:We used transcription activator-like effector nucleases (TALENs) to generate knockout cells for two related microRNAs (miRNAs), mir-141 and mir-200c, which belong to the deeply conserved mir-200 family. By carrying out deep sequencing, we identified the target genes of each miRNA. Interestingly, miR-141 and miR-200c, despite their overall similarity, suppressed largely non-overlapping groups of targets.
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