Transcriptomics

Dataset Information

19

Plant Viral Movement Protein-expressing Yeast Transcriptome compared with Non-Functional Plant Viral Movement Protein-expressing Yeast Transcriptome


ABSTRACT: Virus life cycle heavily depends on their ability to command the host machinery in order to translate their genomes. By using a yeast-based approach we have found that over expression of the PNRSV MP caused a severe growth defect in yeast cells. cDNA microarrays analysis carried out to characterise at the molecular level the growth interference phenotype reported the induction of genes related to amino acid deprivation suggesting that expression of MP activates the GCN pathway in yeast cells. Overall design: Yeast cell cultures were grown 24h in minimal medium with doxycycline (100 mg/ml). These cultures were then diluted to an OD600 nm of 0.1 into fresh minimal medium without doxycycline. At 6 h total RNA was extracted from yeast cells transformed with the pCM:MPpnrsv and MPpnrsvΔHR, a non-functional MPpnrsv mutant lacking its hydrophobic region.

INSTRUMENT(S): Yeast_ALL DNA chip

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Jose Gadea   

PROVIDER: GSE26120 | GEO |

SECONDARY ACCESSION(S): PRJNA135361

REPOSITORIES: GEO

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2014-05-02 | E-GEOD-26120 | ArrayExpress
| GSE26119 | GEO
2014-05-02 | E-GEOD-26119 | ArrayExpress
2014-06-02 | E-GEOD-37905 | ArrayExpress
2014-05-03 | E-GEOD-44871 | ArrayExpress
| GSE107482 | GEO
| PRJNA420340 | ENA
| PRJNA305791 | ENA
| PRJEB10850 | ENA
2010-12-27 | GSE20155 | GEO