Next Generation Sequencing facilitates to identify the differential expression genes of expanded MAIT cells upon the stimulation of 5-OP-RU/MR1 tetramer with/without GCDCA/TCDCA/UDCA

ABSTRACT: We enriched expanded MAIT cells in PBMCs from healthy donor upon the stimulation of 5-OP-RU/MR1 tetramer with/without GCDCA/TCDCA/UDCA through flow sorting to perform next-generation sequencing (NGS). Illumina mRNA-seq sample preparation kit was used to perform paired-end library sequencing with illumina nova seq and sequence analysis was determined using the Illumina data analysis pipeline.

ORGANISM(S): Homo sapiens

PROVIDER: GSE261270 | GEO | 2025/06/01

REPOSITORIES: GEO