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ScComplete-Seq Reveals Comperehensive Picture of Transcriptome

ABSTRACT: Many of the underlying mechanisms of intercellular gene expression heterogeneity are still unknown despite the advancements in single-cell transcriptomics. One of the reasons is the partial detection of non-coding RNA types due to their small size and lack of polyadenylation. The ability to detect gene expression dynamics comprehensively would shed light on the regulation and disease-leading dysregulation in a cell-specific manner, and contribute to the discovery of biomarkers and novel therapeutics. We developed scComplete-seq, a single cell assay that enables concurrent profiling of total RNA. After successful integration of it on available high-throughput single-cell platforms, we validated it on cancer cell lines and applied it to peripheral blood mononuclear cells (PBMCs). This unveiled the distinct regulation of tRNAs, mtRNAs and enhancer RNA in monocytes, T and B cells upon Lipopolysaccharide (LPS) and PMA/Ionomycin stimulation compared to resting cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE261371 | GEO | 2025/07/08

REPOSITORIES: GEO

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