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The comparative methylome analysis of tumor microenvironment from African American and Non- White Hispanic American prostate cancer

ABSTRACT: We investigated differences in DNA methylation in tumor-adjacent stroma (TAS) of African Americans (AA) (n=17) and European Americans (EA) (n=15) to uncover differences that might contribute to a higher rate of aggressive prostate cancer (PCa) in AA men. We performed methyl binding domain sequencing (MBD-seq) on dissected FFPE tumor-adjacent stroma of both cohorts. To further investigate the tumor microenvironment methylome between AA and EA cohorts, we prepared carcinoma associated fibroblasts (CAFs) from fresh prostatectomy tissue of 3 AA and 4 EA patients. Prostate cancer tissues were examined to confirm the location of tumor and a 1-2 mm cube of tumor-adjacent stroma was removed. Frozen stocks of passages 1 to 6 primary cultures were prepared. At passage 6, a portion of the cells were placed in serum-free medium and incubated for 1 day. CAFs were cultured in the presence of PBS or low-dose (0.5 µM) 5AzaC . CAFs were treated with 5AzaC for 1 day, followed by culture for 10 days in drug-free medium.

ORGANISM(S): Homo sapiens

PROVIDER: GSE261520 | GEO | 2025/07/30

REPOSITORIES: GEO

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