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Identification of chromatin at the nuclear periphery in EMS lineage of early C. elegans embryos

ABSTRACT: We performed DNA Adenine Methyltransferase Identification (DamID) to map the contacts between the genome and the nuclear lamina in the EMS lineage of early embryos. Dam::EMR-1 and GFP::Dam fusion proteins were expressed from single-copy transgenes inserted into an intergenic region on chromosome II. To induce a short burst of transcription of the transgenes, we used the med-1 promoter, which is active in the EMS cell of 4 cell-stage embryos and repressed in the descendants of EMS.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE262025 | GEO | 2026/03/01

REPOSITORIES: GEO

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