Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing [Experimentally induced hyperglycemia in chow fed mice]

ABSTRACT: The ARC and DVC are brain region considered to be important in in energey and glucose homesostasis. We used single nucleus RNA sequencing (snRNA-seq) to analyze the response to high fat diet and hyperglycemia.

ORGANISM(S): Mus musculus

PROVIDER: GSE262408 | GEO | 2026/08/24

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing [Experimentally induced hyperglycemia in diet-induced obese mice]

Project description:The ARC and DVC are brain region considered to be important in in energey and glucose homesostasis. We used single nucleus RNA sequencing (snRNA-seq) to analyze the response to high fat diet and hyperglycemia.

2026-08-24 | GSE262407 | GEO

Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing [Experimentally induced hyperglycemia in chow fed mice]

Project description:Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing [Experimentally induced hyperglycemia in chow fed mice]

| PRJNA1091658 | ENA

Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing [Experimentally induced hyperglycemia in diet-induced obese mice]

| PRJNA1091659 | ENA

Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing

Project description:Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing

| PRJNA1091661 | ENA

Endogenous brain ARC complexes

Project description:Arc is an activity regulated neuronal protein yet little is known about its protein interactions, assembly into multiprotein complexes, role in human disease and cognition. We applied an integrated proteomic and genetic strategy using targeted tagging of a Tandem Affinity Purification (TAP) tag and Venus fluorescent protein into the endogenous Arc gene in mice, biochemical and proteomic characterization of native complexes in wild type and knockout mice, and human genetic analyses of disease and intelligence. TAP tagging enabled efficient purification of complexes and identification of many novel Arcinteracting proteins, of which PSD95 was the most abundant. PSD95 was essential for Arc assembly into 1.5 MDa complexes and activity-dependent recruitment to excitatory synapses. Integrating human genetic data with proteomic data showed postsynaptic Arc- PSD95 complexes are enriched in schizophrenia, intellectual disability, autism and epilepsy mutations and normal variants in intelligence. Arc-PSD95 postsynaptic complexes are a molecular substrate for the convergence of normal and pathological genetic variants impacting on human cognitive function.

2017-10-19 | PXD007283 | Pride

Single-cell transcriptomics reveals the effects of high-fat diet on hypothalamic(ARC) and hindbrain(DVC) glucose sensing

Project description:This SuperSeries is composed of the SubSeries listed below.

2026-08-24 | GSE262411 | GEO

Detection of Arc/Arg3.1 oligomers in rat brain: constitutive and synaptic activity-evoked dimer expression in vivo

Project description:The immediate early gene product activity-regulated cytoskeleton-associate protein (Arc or Arg3.1) is a major regulator long-term synaptic plasticity with critical roles in postnatal cortical development and memory formation. However, the molecular basis of Arc function is not defined. Arc is a hub protein with interaction partners in the postsynaptic neuronal compartment and nucleus. In vitro biochemical and biophysical analysis of purified recombinant Arc show formation of low-order oligomers and larger particles including retrovirus-like capsids. Here, we provide evidence for naturally occurring Arc oligomers in mammalian brain. Using in situ protein crosslinking to trap weak Arc-Arc interactions, we identified in various preparations a prominent Arc immunoreactive band on SDS-PAGE of molecular mass corresponding to a dimer. While heavier Arc species or putative trimers and tetramers were detected, they were of lower abundance. In the dentate gyrus (DG) of adult anesthetized rats, induction of long-term potentiation (LTP) by high-frequency stimulation (HFS) of medial perforant synapses or by brief intrahippocampal infusion of BDNF led to a massive increase in Arc dimer expression. Arc immunoprecipitation of crosslinked DG tissue showed enhanced expression of dimer during four hours of LTP maintenance. Mass spectrometric proteomic analysis of immunoprecipitated, gel-excised bands corroborated detection of Arc dimer. Furthermore, Arc dimer was constitutively expressed in naïve cortical, hippocampal and DG tissue, with lowest levels in the DG. Taken together the results implicate Arc dimers as the predominant low-oligomeric form in mammalian brain, exhibiting regional differences in its constitutive expression and pronounced enhanced expression during DG LTP.

2023-07-20 | PXD039612 | Pride

RNA-seq of Arc/Arg3.1-positive versus Arc/Arg3.1-negative skin migratory dendritic cells from healthy mice

Project description:The key function of migratory dendritic cells (migDCs) is to take up antigens in peripheral tissues and migrate to draining lymph nodes (dLN) to initiate immune responses. Recently, we discovered in mice that in the immune system activity-regulated cytoskeleton associated protein/activity-regulated gene 3.1 (Arc/Arg3.1) is exclusively expressed by migDCs and is a central driver of fast inflammatory migration. However, the frequency of Arc/Arg3.1 expressing cells in different migDC subsets including Langerhans cells (LC), their phylogenetic origin, transcription factor dependency and functional role remains unclear. Here we found that Arc/Arg3.1+ migDCs derived from common DC precursors (CDPs) and radio-resistant LCs. They were present in all migDC subsets and showed a consistent superiority in inflammatory migration but were independent of the transcription factors Irf4 or Batf3. In intradermal Staphylococcus aureus infection, a model that relies on effective inflammatory antigen transport, Arc/Arg3.1 deletion strongly reduced T cell responses. By contrast, Arc/Arg3.1 deficiency did not hamper the immune response to systemic Listeria monocytogenes infection, which does not require antigen transport. Thus, our results show that Arc/Arg3.1 is a molecular marker for defining a population of DCs with superior migratory capacity that spans across all migDC subsets irrespective of ontogeny and phenotype.

2021-06-22 | GSE116161 | GEO

High-resolution mapping of regulatory element interactions and genome architecture using ARC-C [ARC-C]

Project description:Interactions between cis-regulatory elements such as promoters and enhancers are important for transcription but global identification of these interactions remains a major challenge. Leveraging the chromatin accessiblity of regulatory elements, we developed ARC-C (accessible region chromosome conformation capture), which profiles chromatin regulatory interactions genome-wide at high resolution. Applying ARC-C to C. elegans, we identify ~15,000 significant interactions at 500bp resolution. Regions bound by transcription factors and chromatin regulators such as cohesin and condensin II are enriched for interactions, and we use ARC-C to show that the BLMP-1 transcription factor mediates interactions between its targets. Investigating domain level architecture, we find that C. elegans chromatin domains defined by either active or repressive modifications form topologically associating domains (TADs) and that these domains interact to form A/B (active/inactive) compartment structure. ARC-C is a powerful new tool to interrogate genome architecture and regulatory interactions at high resolution.

2021-06-01 | GSE144670 | GEO

ARC-SSMS-green-tea-metabolomics

Project description:green tea extracts analyzed with DDA MS for self service metabolomics training in the ARC MS facility at CSU

2023-02-09 | MSV000091238 | MassIVE