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A comprehensive atlas of testicular interstitium reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitors [scRNA-Seq]

ABSTRACT: Decreasing fertility rates has emerged as a significant social and medical concern, with male infertility accounting for at least half of the cases. Conventional semen analysis offers restricted prognostic utility for male fertility, largely because a considerable number of male infertility incidents are attributed to dysfunctions in the testicular interstitium. Here we have not only characterized transcriptome data and reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitor, but also examined super enhancers information in both young, adult and aged mice Leydig cell progenitors. Our findings reveal A comprehensive atlas of testicular interstitium during aging in both H3K27ac modifications and gene expression alterations. Specifically, the transcriptional activities of key genes involved in progenitor stemness appear to be regulated by super-enhancers. These discoveries offer pivotal insights for developing novel cell-based therapies to ameliorate testicular dysfunction in older individuals.

ORGANISM(S): Mus musculus

PROVIDER: GSE262416 | GEO | 2026/01/21

REPOSITORIES: GEO

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A comprehensive atlas of testicular interstitium reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitors [CUT&Tag]

Project description:Decreasing fertility rates has emerged as a significant social and medical concern, with male infertility accounting for at least half of the cases. Conventional semen analysis offers restricted prognostic utility for male fertility, largely because a considerable number of male infertility incidents are attributed to dysfunctions in the testicular interstitium. Here we have not only characterized transcriptome data and reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitor, but also examined super enhancers information in both young, adult and aged mice Leydig cell progenitors. Our findings reveal A comprehensive atlas of testicular interstitium during aging in both H3K27ac modifications and gene expression alterations. Specifically, the transcriptional activities of key genes involved in progenitor stemness appear to be regulated by super-enhancers. These discoveries offer pivotal insights for developing novel cell-based therapies to ameliorate testicular dysfunction in older individuals.

2026-01-21 | GSE262415 | GEO

NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [multiome]

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NR2F2 Regulation of Fetal Leydig Cell Differentiation: Insights into Differences of Sex Development [RNA-Seq]

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Project description:Testicular fetal Leydig cells are specialized for androgen production during embryogenesis. Testosterone is essential for regulating sex differentiation, spermatogenesis, and fertility. Deficiencies in Leydig cell differentiation can lead to various disorders of sex development and male reproductive conditions, such as ambiguous genitalia, hypospadias, cryptorchidism, and infertility. Understanding the differentiation of fetal Leydig cells is essential for comprehending male sexual differentiation, reproductive health, and fertility. Fetal Leydig cells originate from proliferating progenitor cells in the gonadal interstitium, marked by genes like Arx, Pdgfra, Tcf21, Wnt5a, and Nr2f2 (COUP-TFII). However, the precise mechanisms governing the transition from interstitial cells to Leydig cells remain elusive. Through integrated approaches involving animal models and multiomics, we have demonstrated that fetal Leydig cells originate from a COUP-TFII positive non-steroidogenic interstitial cell population. COUP-TFII promotes progenitor cell fate while suppressing Leydig cell differentiation. Moreover, embryonic deletion of COUP-TFII in mouse testes resulted in disorders of sex development, including reduced testicular size, Leydig cell hypoplasia, cryptorchidism, and hypospadias. Collectively, our findings highlight the critical role of COUP-TFII in orchestrating the transition from interstitial cells to Leydig cells during testicular development.

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A comprehensive atlas of testicular interstitium reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitors [scRNA-Seq]

Project description:A comprehensive atlas of testicular interstitium reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitors [scRNA-Seq]

| PRJNA1091684 | ENA

A comprehensive atlas of testicular interstitium reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitors [CUT&Tag]

Project description:A comprehensive atlas of testicular interstitium reveals Cd34+/Sox4+ mesenchymal cells as potential Leydig cell progenitors [CUT&Tag]

| PRJNA1091682 | ENA

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