Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways [scRNA-seq]

ABSTRACT: We performed single cell RNA sequencing to investigate differentially expressed genes in each airway cell type after Cdh1 inactivation in Club cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE262858 | GEO | 2025/03/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-03-26 | GSE263032 | GEO

E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways [RNA-seq]

Project description:E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways

2025-03-26 | GSE256496 | GEO

E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways [scRNA-seq]

Project description:E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways [scRNA-seq]

| PRJNA1093558 | ENA

E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways

Project description:E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways

| PRJNA1095476 | ENA

Radiation-activated secretome of Scgb1a1+ club cells increase the efficacy of immune checkpoint blockade in lung cancer (iDTR scRNA-seq)

Project description:We report that airway epithelial club cells affected the immune landscaping in HKP1 lungs

2021-06-08 | GSE157882 | GEO

The NOTCH3 Downstream Target HEYL Is Required for Efficient Human Airway Basal Cell Differentiation [scRNA-Seq]

Project description:Basal cells (BC) are the resident stem/progenitor cells of the adult pseudostratified airway epithelium, whose differentiation program is orchestrated by the NOTCH signaling pathway. NOTCH3 receptor mediated signaling regulates BC to club cell differentiation; however, the downstream responses that regulate this process are largely unknown. In the present study we utilized an in vitro air-liquid interface model of the human pseudostratified airway epithelium to identify the NOTCH3-dependent downstream genes/pathways that regulate human BC to club cell differentiation. Activation of NOTCH3 signaling in BC via lentivirus-mediated over-expression of the active NOTCH3 intracellular domain (NICD3) promoted club cell differentiation. Bulk RNA-seq analysis of control vs NICD3 -transduced cells, identified 692 NICD3 responsive genes enriched for pathways linked to airway epithelial biology and differentiation including Wnt/β-catenin Signaling. Expression of the classical NOTCH target HEYL increased in response to NOTCH3 activation and positively correlated with the club cell marker SCGB1A1. Further, using single-cell RNA-seq, we report that HEYL+ cells primarily clustered with SCGB1A1+ and NOTCH3+ cells. Moreover, HEYL protein co-localized with SCGB1A1 in ALI cultures in vitro and in the human and mouse airway epithelium in vivo. siRNA-mediated knockdown of HEYL in BC led to changes in epithelial structure including altered morphology and significant reductions in transepithelial electrical resistance and expression of tight junction related genes. Finally, HEYL knockdown significantly reduced the number of SCGB1A1+ club cells, along with a corresponding increase in KRT8+ BC-intermediate cells. Overall, our data identifies NOTCH3-HEYL signaling as a key regulator of BC to club cell differentiation.

2021-03-04 | GSE168127 | GEO

Radiation-activated secretome of Scgb1a1+ club cells increase the efficacy of immune checkpoint blockade in lung cancer (0Gy4Gy scRNA-seq)

Project description:We report that airway epithelial club cells harbor the genes specifically up-regulated by 4Gy-RT

2021-06-08 | GSE157881 | GEO

E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways [RNA-seq]

Project description:E-cadherin has a cell-type specific role in maintaining the integrity of pulmonary airways [RNA-seq]

| PRJNA1079788 | ENA

Modeling, dissecting, and subtyping of E-cadherin inactivation-associated diffuse-type gastric adenocarcinoma

Project description:Diffuse-type gastric adenocarcinoma (DGAC) is lethal cancer that is often diagnosed late and resistant to therapeutics. Although hereditary DGAC is mainly characterized by mutations in the CDH1 gene that encodes E-cadherin, the impact of E-cadherin inactivation in DGAC tumorigenesis remains unclear. To address this, we established and characterized a CDH1 loss-associated DGAC model system with a human DGAC single-cell transcriptome. We genetically engineered murine gastric organoids (GOs; Cdh1 KO, KrasG12D, Trp53 KO [EKP]) that recapitulates human DGAC tumorigenesis. Cdh1 depletion is sufficient to 1. Single-cell transcriptomics of human DGAC datasets classified patients into three subtypes (DGAC1, 2, and 3). By transcriptional signature, EKP GOs belong to the DGAC1 subtype displaying CDH1 downregulation and epithelial-mesenchymal transition. Compared to DGAC2 and DGAC3, the DGAC1 subtype was characterized by T cell exhaustion, PILRA-CD99 enrichment, and potential sensitivity to PD1 inhibitors. Additionally, we identified the EZH2-mediated transcriptional circuit as a key regulon specifically activated in EKP and a therapeutic vulnerability. This study unravels the unexpected role of E-cadherin loss in cell lineage plasticity, transcriptional reprogramming, and immune evasion of DGAC and further stratifies DGAC patients by single-cell transcriptomics, providing novel insights into E-cadherin loss-associated DGAC tumorigenesis.

2023-08-07 | GSE226266 | GEO

Biology and Ontogeny of Human Small Airway Epithelium Club Cells Identified with Single Cell Transcriptome and Principle Component Gradient Analysis

Project description:Club (“Clara”) cells, dome shaped cells with disease cytoplasmic granules and microvilli, are the major secretory cell of the human small (>6 generation) airways. Little is known regarding the biology of club cells in the human airway, nor of the ontogeny of this cell type. Taking advantage of the SCGB1A1 as the marker for club cells and our ability to sample the normal small airway epithelium by bronchoscopy and brushing healthy volunteers, we defined the transcriptome of the normal human airway club cell using single cell transcriptome sequencing. Analysis of the human small airway epithelial single cell transcriptome together with in vitro validation provides novel insights into the molecular phenotype and biological functions of the human club cell population and identifies the basal cell as the human progenitor cells for club cells.

2024-06-05 | GSE94819 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data