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Deep sequencing analysis of the murine transcriptome response to different virulent parenthood B. melitensis infection

ABSTRACT: In this study, we employed the Illumina Genome Analyzer platform to perform a Digital Gene Expression (DGE) analysis of the peritoneal macrophages genome-wide transcriptome response to B.melitensis infection. Common changes in gene expression were observed among Brucella app. infected macrophages suggesting similar strategies were employed for their survival and replication, inducing anti-inflammatory and anti-apoptotic. A total of 1019 differentially expressed (DE) transcripts were identified in the macrophages 4 hours after differ virluent B.melitensis infection, especially genes participated lysosome pathway and MAPK pathway. Our findings demonstrate previously unrecognized changes in gene transcription that are associated with B.melitensis infection in the macrophages, and many significant pathways (cascades) identified in the study clearly merit further investigation. Our data provide new clues to understand the molecular attenuation mechanism of M5-90 and a firm foundation for reducing vaccine residual virulence, enhancing vaccine efficacy.

ORGANISM(S): Mus musculus

PROVIDER: GSE26337 | GEO | 2012/12/01

SECONDARY ACCESSION(S): PRJNA136913

REPOSITORIES: GEO

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Deep sequencing analysis of the murine transcriptome response to different virulent parenthood B. melitensis infection

Project description:In this study, we employed the Illumina Genome Analyzer platform to perform a Digital Gene Expression (DGE) analysis of the peritoneal macrophages genome-wide transcriptome response to B.melitensis infection. Common changes in gene expression were observed among Brucella app. infected macrophages suggesting similar strategies were employed for their survival and replication, inducing anti-inflammatory and anti-apoptotic. A total of 1019 differentially expressed (DE) transcripts were identified in the macrophages 4 hours after differ virluent B.melitensis infection, especially genes participated lysosome pathway and MAPK pathway. Our findings demonstrate previously unrecognized changes in gene transcription that are associated with B.melitensis infection in the macrophages, and many significant pathways (cascades) identified in the study clearly merit further investigation. Our data provide new clues to understand the molecular attenuation mechanism of M5-90 and a firm foundation for reducing vaccine residual virulence, enhancing vaccine efficacy. Examination oftwo peritoneal macrophages infected brucella and one blank control.

2012-12-01 | E-GEOD-26337 | biostudies-arrayexpress

Transcriptome analysis reveals the effect of STAT3 gene of JAK-STAT signaling pathway on apoptosis of Brucella macrophages

Project description:Brucella is a significant intracellular parasitic bacterium that establishes an infection within a host cell to facilitate its proliferation. While the survival of Brucella in cells is an important manifestation of its virulence, the research on this aspect is not yet complete. Transcriptome sequencing techniques were employed for the analysis of transcript levels of various genes in RAW 264.7 macrophages infected with M5 and wild strains. siRNA can be employed to silence differentially expressed genes, with the subsequent validation of their functions conducted through a variety of techniques, including RT-qPCR, Western blot, flow cytometry, and others.In the study, a significant up-regulation of the STAT3 gene was observed in the vaccine strain when compared with the wild strain. The design of the relevant siRNA to silence STAT3 resulted in the promotion of apoptotic gene expression and the inhibition of apoptosis-inhibiting gene expression, thereby facilitating cell apoptosis.The results demonstrated that the discrepancy in STAT3 gene transcription between the vaccine and wild strains resulted in a reduction in cell damage and a decline in the virulence of the vaccine strain M5. This finding offers novel insights into the intracellular survival mechanism of Brucella and the underlying cause of M5 weakness.

2024-12-13 | GSE284112 | GEO

Brucella melitensis M5 strain:M5

Project description:Brucella melitensis vaccine strain M5 Genome sequencing

| PRJNA187210 | ENA

Brucella melitensis M5-10

Project description:Brucella melitensis Vaccine Strain M5-10 Genome Sequencing

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The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by b. melitensis m5-90 [miRNA]

Project description:We used B. melitensis M5-90 wild type to construct gene-deleted strains of B. melitensis ∆per, RAW264.7 cells infected with B. melitensis M5-90 and B. melitensis M5-90 ∆per for 4h, respectively. miRNA microarray and mRNA array experiments were performed, qRT-PCR validation for miRNAs and mRNAs. We performed a joint analysis of differentially expressed miRNAs and mRNAs, and proved that the target gene of miRNA-146b is Tbc1d14. It further confirmed that miR-146b targeting tbc1d14 regulates brucella-mediated autophagy of RAW264.7 cells. Finally, the molecular mechanism of tbc1d14 influencing brucella-mediated autophagy of RAW264.7 cells was preliminarily revealed by DGE sequencing.

2019-02-14 | GSE126498 | GEO

The role of mirna-146b-tbc1d14 in the autophagy of RAW264.7 cells mediated by B. melitensis m5-90 [mRNA]

2019-02-11 | GSE126343 | GEO

Key hub and bottleneck genes differentiate the macrophage response to virulent and attenuated Mycobacterium bovis

Project description:Mycobacterium bovis is an intracellular pathogen that causes tuberculosis in cattle. Following infection, the pathogen resides and persists inside host macrophages by subverting host immune responses via a diverse range of mechanisms. Here, a high-density bovine microarray platform was used to examine the bovine monocyte-derived macrophage transcriptome response to M. bovis infection relative to infection with the attenuated vaccine strain, M. bovis Bacille CalmetteM-bM-^@M-^SGuM-CM-)rin. Differentially expressed genes were identified (adjusted P-value M-bM-^IM-$ 0.01) and interaction networks generated across an infection time course of 2, 6 and 24 h. The largest number of biological interactions was observed in the 24 h network, which exhibited small-worldscale-free network properties. The 24 h network featured a small number of key hub and bottleneck gene nodes, including IKBKE, MYC, NFKB1 and EGR1 that differentiated the macrophage response to virulent and attenuated M. bovis strains, possibly via the modulation of host cell death mechanisms. These hub and bottleneck genes represent possible targets for immunomodulation of host macrophages by virulent mycobacterial species that enable their survival within a hostile environment. Affymetrix GeneChipM-BM-. Bovine Genome Arrays were used to examine gene expression from a paired comparison of bovine monocyte-derived macrophages (MDM) after in vitro challenge with Mycobacterium bovis versus M. bovis BCG across a time series of 2 hr, 6 hr and 24 hr post-challenge.

2014-08-01 | E-GEOD-59774 | biostudies-arrayexpress

Key hub and bottleneck genes differentiate the macrophage response to virulent and attenuated Mycobacterium bovis

Project description:Mycobacterium bovis is an intracellular pathogen that causes tuberculosis in cattle. Following infection, the pathogen resides and persists inside host macrophages by subverting host immune responses via a diverse range of mechanisms. Here, a high-density bovine microarray platform was used to examine the bovine monocyte-derived macrophage transcriptome response to M. bovis infection relative to infection with the attenuated vaccine strain, M. bovis Bacille Calmette–Guérin. Differentially expressed genes were identified (adjusted P-value ≤ 0.01) and interaction networks generated across an infection time course of 2, 6 and 24 h. The largest number of biological interactions was observed in the 24 h network, which exhibited small-worldscale-free network properties. The 24 h network featured a small number of key hub and bottleneck gene nodes, including IKBKE, MYC, NFKB1 and EGR1 that differentiated the macrophage response to virulent and attenuated M. bovis strains, possibly via the modulation of host cell death mechanisms. These hub and bottleneck genes represent possible targets for immunomodulation of host macrophages by virulent mycobacterial species that enable their survival within a hostile environment.

2014-08-01 | GSE59774 | GEO

Comparative transcriptomic analysis of THP‑1 derived macrophages infected with Mycobacterium tuberculosis H37Rv, H37Ra and BCG

Project description:In this study, RNA sequencing (Transcriptome sequencing) was employed to investigate the global transcriptome changes in the macrophages during the different strains of M.tb infection. THP-1 cells derived macrophages were exposed to the virulent M.tb strain H37Rv (Rv) or the avirulent M.tb strain H37Ra (Ra), and the M.tb BCG vaccine strain was used as a control. The cDNA libraries were prepared from M.tb infected macrophages and then sequenced.

2020-12-06 | GSE162729 | GEO

Immunological responses unique to virulent F. tularensis Schu4 in the mouse model of infection

Project description:Francisella tularensis is a category A select agent based on its infectivity and virulence but disease mechanisms in F. tularensis infection remain poorly understood. A murine pulmonary model of infection was therefore employed to characterize the host immune response to F. tularensis infection and to discern differences in responses to infection with the highly virulent Type A F. tularensis strain Schu4 and to the less virulent Type B live vaccine strain (LVS). Mice were infected intranasally with F. tularensis Schu4 or LVS and organ lesions were assessed 48 and 120 hours after infection. Experiments were also conducted to assess and compare the host immune response to infection using global transcriptional analysis. Mice were infected via low dose aerosol with F. tularensis Schu4 or LVS strains, and transcriptional response in the lungs and spleen was monitored using full mouse genome microarrays at 12, 24, 48, and 120 hours after infection. We found differential and temporal differences in expression of cytokine and chemokine genes, CD molecules, apoptosis, leukocytes and adhesion receptors, and immunological signaling between infection with Schu4 and LVS strains. The transcriptional differences coincided with marked differences in the timing and extent of organ lesions in mice infected with the LVS and Schu4 strains. Thus, these studies revealed both temporal and qualitative differences in the host immune response to infection with virulent F. tularensis Schu4 compared to infection with LVS.

2010-11-01 | GSE22203 | GEO

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