Transcriptomics

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Gene expression profile at single embryo level of 0-3h old hybrid Drosophila embryos


ABSTRACT: We used single-embryo RNA-sequencing (RNA-seq) to characterize early zygotic gene expression in Drosophila. To assess the accurate transcriptional activation for zygotic genes, we analyze the paternal allele expression as embryos initially contain trace amounts of paternal mRNAs delivered by the sperm. For this purpose, we used two genetically different lines from the Drosophila Genetic Reference Panel (DGRP) with known genetic variations in our crosses (males/DGRP_352, females/DGRP_737). The data provide a continuous timeline of transcript levels and allele-specific gene expression during early development (≤ 3 hours) in Drosophila melanogaster. Deposited RNA-seq data is part of a multi-'omics approach and prior metabolite extraction was performed in each sample.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE263568 | GEO | 2025/07/03

REPOSITORIES: GEO

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