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Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (gene expression)

ABSTRACT: Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive.

ORGANISM(S): Homo sapiens

PROVIDER: GSE26377 | GEO | 2012/02/02

SECONDARY ACCESSION(S): PRJNA142277

REPOSITORIES: GEO

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Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (miRNA)

2012-02-02 | GSE26376 | GEO

Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (gene expression)

Project description:Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive. After treatment of TPA, U937 cells present functional differentiation markers as well as specific cell morphology, which were confirmed by flow cytometric analysis and Wright-Giemsa staining.Here we use genome-wide microarray analysis to profile the expression levels of mRNA in normal U937 cells and differentiated U937 cells. For each differentiated and control sample, two hybridizations were performed by using a reversal fluorescent strategy.

2012-02-02 | E-GEOD-26377 | biostudies-arrayexpress

Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (miRNA)

Project description:Since the expression profile of miRNAs is specific in different tissues or under different physiological conditions, the correlations between miRNAs and mRNAs could vary under different biological circumstances. This is a study also used expression profiles of miRNA and mRNA during monocytic differentiation to explore the correlations between the expression levels of miRNAs and mRNA, either negative or positive. After treatment of TPA, U937 cells present functional differentiation markers as well as specific cell morphology, which were confirmed by flow cytometric analysis and Wright-Giemsa staining.Here we use a miRNA microarray (CapitalBio, Beijing, China) containing 509 well-characterized human, mouse and rat miRNAs and various controls to profile the expression levels of miRNA in normal U937 cells and differentiated U937 cells. Three independent biological experiments were performed, and for each differentiated and control sample, two hybridizations were performed by using a reversal fluorescent strategy.

2012-02-02 | E-GEOD-26376 | biostudies-arrayexpress

Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation

Project description:This SuperSeries is composed of the following subset Series: GSE26376: Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (miRNA) GSE26377: Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation (gene expression) Refer to individual Series

2012-02-02 | E-GEOD-26379 | biostudies-arrayexpress

Microarray profiling of monocytic differentiaion reveals miRNA-mRNA intrinsic correlation

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-02-02 | GSE26379 | GEO

Monocytic U937 cells and receptor cross-linking

Project description:Monocytic U937 cell lines and crosslinked CD32 & CD64 at different time points Keywords: time-course

2009-07-20 | GSE1537 | GEO

Induction of microRNA expression and processing via ERK/MAPK signaling during monocytic differentiation

Project description:This study produced a microarray-based screening for miRNAs responding to tetradecanoyl phorbol acetate (TPA) (for monocytic differentiation) treatment in various leukemia cell lines.

2013-12-01 | GSE33537 | GEO

Transcrription profiling of human monocytic U937 cells and receptor cross-linking

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Gene expression profile of macrophages of monocytic origin and Kupffer cells under conditions of co-cultivation with Ito cells, endotheliocytes of sinusoidal capillaries of the liver and HGF.

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2023-07-05 | GSE236447 | GEO

MitomiR profiling of mitochondria extracted from monocytic cell line

Project description:MiRNAs play pivotal roles in regulating macrophage functions, including differentiation, polarization, recruitment, and activation of inflammation. in this review, to provide novel insight into macrophage differentiation-specific mitomiR signatures and associated mitochondrial pathways, we performed a global miRNA profiling on extracted mitochondria and total cell content of human macrophages. The THP-1 monocytic cell line was treated with phorbol-12-myristate-13-acetate (PMA) to induce differentiation into macrophages The human monocytic cell line THP-1 cells were differentiated into THP-1 derived macrophages with 40 ng/mL phorbol 12-myristate 13-acetate (PMA),The mitochondrial fraction of the cells were enriched and purified using anti-TOM22 microbeads (Miltenyi Biotec). After total RNA extraction, and using Taqman low-density arrays (TLDA), we performed the global miRNA profiling on extracted mitochondria and compared with total cell content

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