Regulation of Microprocessor assembly and localization via Pasha’s WW domain in C. elegans

ABSTRACT: Primary microRNA (pri-miRNA) transcripts are processed by a protein complex called the Microprocessor comprised of the ribonuclease Drosha and its RNA binding partner DGCR8/Pasha. We sequenced small RNAs from animals containing a mutation in the WW domain of C. elegans pash-1. We found that these mutants have a modest but widespread reduction in miRNA levels when grown at 20˚C, which is further enhanced when grown at 25˚C. The results demonstrate a requirement for the WW domain in processing miRNAs in C. elegans.

ORGANISM(S): Caenorhabditis elegans

PROVIDER: GSE263914 | GEO | 2024/04/16

REPOSITORIES: GEO