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Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE264037 | GEO | 2026/06/04

REPOSITORIES: GEO

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Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism [RNA-seq]

Project description:Embryo implantation coincides with the Naïve-to-Primed pluripotency transition. From fertilization to implantation only a number of cleavages is observed without significant embryo growth. Embryo-uterine connection provides necessary nutrients for further development while metabolism intensification is occurred. We have found that in vitro Naïve-to-Primed pluripotency transition of ESCs with Pcbp1 knockout is worsened – reduction of proliferation and cell death is observed. By using ChIP-seq, RNA-seq and Mass-spectrometry we show that Pcbp1 is detrimental for pluripotent properties, but is crucial for amino acid metabolism intensification. According to this, functional approaches showed protein synthesis decline, while embryos deficient for Pcbp1 demonstrated growth absence after implantation.

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Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism [ChIP-seq]

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Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism

Project description:Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism

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Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism [ChIP-seq]

Project description:Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism [ChIP-seq]

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Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism [RNA-seq]

Project description:Pcbp1 is crucial for Naïve-to-Primed pluripotency transition due to regulation of amino acid metabolism [RNA-seq]

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Pcbp1 blocks MEK/ERK signaling and facilitate Naïve to Primed pluripotency

Project description:Embryonic stem cells (ESCs) represent a unique cell type that is characterized by the ability to self-renew and differentiate into all types of somatic cells, as well as into germ cells. An understanding of the mechanisms which underlie their functioning is necessary for their usage in regenerative medicine. Pcbp1 is a DNA/RNA poly(C)-binding protein with a broad spectrum of functions including transcription, mRNA processing, and alternative splicing. In this study, we demonstrate that Pcbp1 blocks spontaneous ESCs differentiation towards primitive endoderm (PrE) by fine-tuning MEK/ERK signaling. Knockout of Pcbp1 leads to a specific PrE Oct4+Gata6+ population which is not receptive to differentiation signals. MEK/ERK inhibition prevents this phenotype but as our RNA-seq and Mass-spec data show, further Naïve-to-Primed transition is failed and leads to cell death, which is caused by the conflict of Primed and PrE gene expression network. Our results could explain an early lethal phenotype of Pcbp1-knockout mouse embryos and make clear the affected mechanisms. This dataset represents shotgun proteomics comparison of Mus musculus ESCs in Naïve to Primed states with or without Pcbp1 mutation.

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Cpt1a drives Lipid remodeling in Primed-to-Naïve Pluripotency Transition

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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