Co-evolution of esophageal cancer cells and microenvironment mediate resistance to chemoradiation therapy

ABSTRACT: Understanding how cancer cells and their tumor microenvironment (TME) mediate chemoradiation (CRT) resistance in esophageal adenocarcinoma (EAC) is key for improving the low treatment response rate in the clinic. Here, we generated single cell transcriptomics data of 302,918 cells from N=84 longitudinal samples that was used to identify TME and cancer cell programs associated with treatment responses along the course of CRT. Our data showed that interferon-activated immune cells acquired higher tumor-reactivate phenotypes. We also found a large expansion of myeloid cells with different infiltration frequencies between the good-responders and non-responders during and after CRT. Furthermore, we identified four TME ecotypes and four cancer expression subtypes that were strongly associated with CRT responses. This data was used to construct a prognostic 12-gene panel that stratified patients into clinically meaningful survival subgroups. Collectively, our data provided valuable insights into the biology of EAC CRT responses and identified novel predictive biomarkers and actionable targets to enhance CRT efficacy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE264077 | GEO | 2024/05/19

REPOSITORIES: GEO