Project description:To compare the transcriptomic profile of mouse spinal cord homegenates from different conditions using the NanoString nCounter Mouse Glial Profiling Panel with additional customized probes.
Project description:CD73ko and control mice were subjected to transient middle cerebral artery occlusion (tMCAO) for 50min or subjected to equally long sham surgery. Whole brain RNA was isolated and analyzed with the NanoString nCounter® mouse glial profiling panel.
Project description:As a rapid Stem11 scoring platform, a custom NanoString nCounter panel was designed. Alongside the Stem11 genes, the previously published LSC17 genes were included as an established transcriptional prognostic system for AML. For data normalization, seven housekeeping genes were further included from a previous custom NanoString nCounter panel. The full list of the 35 genes in our custom panel is provided in the manuscript.
