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METTL3 regulates microglial inflammation through the m6A modification

ABSTRACT: Microglia, the most abundant type of brain-resident immune cells, continuously surveil the environment and play a central role in shaping the inflammatory state of the central nerve system (CNS). We discovered that the protein expression of METTL3 (a m6A methyltransferase) were up-regulated in inflammatory microglia. We conducted MeRIP-seq analyses in LPS-stimulated microglial cells with or without METTL3 knockout. In the MeRIP-seq analysis, the m6A modifications are primarily located in a consensus “RRACH” motif, and m6A peaks mainly occur in coding sequences (CDSs), 3′ untranslated regions (3′ UTRs), and near stop codons.Further study identified that METTL3 promoted the expression of Basic Leucine Zipper Transcriptional Factor ATF-Like (BATF), which in turn directly bound to a cohort of characteristic inflammatory cytokines and chemokine genes. Collectively, our findings identified METTL3-m6A-BATF axis as a potential therapeutic target for terminating detrimental microglial inflammation

ORGANISM(S): Mus musculus

PROVIDER: GSE264486 | GEO | 2025/04/20

REPOSITORIES: GEO

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