Transcriptomics

Dataset Information

20

Expression analysis of Mycobacterium smegmatis delta-MSMEG_0166 mutant


ABSTRACT: Investigation of whole genome gene expression level changes in a Mycobacterium smegmatis mc2 155 delta-MSMEG_0166 mutant, compared to the wild-type strain. MSMEG_0166 is a transcriptional regulator in the gntR family. Mutations in MSMEG_0166 result in hypersensitivity to the bactericidal ubiquitin peptide Ub2, as well as oxidative stress caused by either organic or inorganic agents Overall design: A three chip study using total RNA recovered from three separate wild-type cultures of Mycobacterium smegmatis mc2 155 and three separate cultures of a MSMEG_0166 mutant strain, Mycobacterium smegmatis mc2 155 delta-MSMEG_0166, in which MSMEG_0166 is interupted by the insertion of a hygromycin resistance cassette. Each chip measures the expression level of 6,588 genes from Myobacterium smegmatis with five 60-mer probes per transcript and two replicates of each probe for a total of 65,880 experimental probes.

INSTRUMENT(S): NimbleGen Mycobacterium smegmatis 6.7k array [100415_Msme_GP_exp]

ORGANISM(S): Mycolicibacterium smegmatis MC2 155  

SUBMITTER: Melissa Suzan Standley  

PROVIDER: GSE26474 | GEO | 2011-01-06

SECONDARY ACCESSION(S): PRJNA136523

REPOSITORIES: GEO

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