Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Response of ein3eil1 mutants to fe deficiency-Role of ethylene in Fe deficieny response signalling

ABSTRACT: au10-14_fer - response of ein3eil1 mutants to fe deficiency - Response of ein3eil1 mutants to Fe deficiency - Wild type seedlings and ethylene insensitive ein3eil1 seedlings were germinated and grown in the presence of 50 µM Fe or absence of Fe (0 µM) on Hoagland medium agar plates until the age of 6 days. Under these growth conditions symptoms of Fe deficiency develop in the 0 Fe plants. Ethylene is known to promote Fe acquisition responses. Whole seedlings were harvested for transcriptome analysis, in a total of three biological replicates.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE26510 | GEO | 2011/12/31

SECONDARY ACCESSION(S): PRJNA136691

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

response of ein3eil1 mutants to fe deficiency-Role of ethylene in Fe deficieny response signalling

Project description:au10-14_fer - response of ein3eil1 mutants to fe deficiency - Response of ein3eil1 mutants to Fe deficiency - Wild type seedlings and ethylene insensitive ein3eil1 seedlings were germinated and grown in the presence of 50 µM Fe or absence of Fe (0 µM) on Hoagland medium agar plates until the age of 6 days. Under these growth conditions symptoms of Fe deficiency develop in the 0 Fe plants. Ethylene is known to promote Fe acquisition responses. Whole seedlings were harvested for transcriptome analysis, in a total of three biological replicates. 12 dye-swap - gene knock out,treated vs untreated comparison

2011-12-31 | E-GEOD-26510 | biostudies-arrayexpress

Comparison 39ox and wt samples with or without fe-bHLH039 overexpression results in iron accumulation dependent on the FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT)

Project description:au13-07_ox - comparison 39ox and wt samples with or without fe - Differential gene expression between 39Ox and WT at +Fe and -Fe - WT (Col-0) and 39Ox seeds were surface sterilized and grown directly on +Fe or -Fe Hoagland medium for 6 days. 60 whole seedlings were harvested in liquid nitrogen and grinded to a fine powder. Total RNA was extracted with the Spectrum Plant total RNA kit (Sigma-Aldrich). 8 µg total RNA was sent to analysis. For validation of the results, RT-qPCR was performed using the same RNA samples.

2017-02-01 | GSE93093 | GEO

Col-0 anthers, control and iron-deficient

Project description:RNAseq transcriptome of anthers of Arabidopsis thaliana Columbia-0 grown under control (1/2 Hoagland) and Fe deficiency conditions.

2021-07-31 | GSE153588 | GEO

Salicylic acid accumulation is required for Fe-deficiency responses in Arabidopsis

Project description:Several phytohormones and other small molecules have been demonstrated to be involved in iron (Fe) homeostasis. However, how salicylic acid (SA), an essential hormone in plant immunity and defense responses, participates in Fe-deficiency responses in plants is largely unknown. Here, we took advantage of a SA biosynthesis defect mutant phytoalexin deficient 4 (pad4: T-DNA Salk_089936) to explore the possible effects of endogenous SA on the morphological and physiological responses to Fe deprivation. Under a Fe-deficiency treatment, Col-0 showed more severe leaf chlorosis and root growth inhibition compared with the pad4 mutant. The soluble Fe concentrations were significant higher in pad4 than Col-0 under the Fe-deficiency treatment, suggesting that a mutation in the PAD4 gene may alleviate the Fe-deficiency-induced symptoms by regulating the soluble Fe concentrations. Furthermore, a SA signaling maker line (PR1promoter: GUS) was used to investigate how Fe deficiency affects endogenous SA biosynthesis and metabolism. The data showed that Fe deficiency significantly induced SA accumulation in Col-0, and the loss function of PAD4 blocked this process. The requirement of endogenous SA accumulation for Fe-deficiency responses was confirmed using a series of SA biosynthetic mutants and transgenic lines.

2016-10-01 | GSE74515 | GEO

Differential expression for P and Fe

Project description:Root samples were used We exposed young seedlings to P and Fe deficiency

2016-01-01 | GSE52159 | GEO

fe-fit-diff_6d-Differential gene expression in A. thaliana roots depending on iron supply and FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) abundance

Project description:au15-01_iron-fit - fe-fit-diff_6d - Changes in gene expression profiles between fit knock-out, wild-type and FIT overexpressor seedlings under sufficient iron supply and under iron deficiency. - Col-0, HA-FIT and fit-3.

2016-07-01 | GSE80281 | GEO

Col-0 leaves and root, control and iron-deficient

Project description:RNAseq transcriptome of leaves and roots of Arabidopsis thaliana Columbia-0 grown under control (ES media) and Fe-deficiency (-Fe +100 µM FRZ) conditions.

2016-10-08 | GSE87760 | GEO

RNA-seq analysis of Arabidopsis roots of Col-0, fls2 and UBQ10::mCitrine-IMA1 in response to +Fe, +Fe+flg22, -Fe and -Fe+flg22

Project description:We reported the flg22-triggered immune responses in roots affect the iron deficiency responses and may link to the function of FLS2 and IMA1 in the root. To identify the underlying mechanism of how the root transcriptome profiles respond to +Fe, +Fe+flg22, -Fe, -Fe+flg22 respectively, and if the flg22 responses is dependent on the function of FLS2 and IMA1 in the root, we performed an mRNA-seq experiments in Col-0, fls2 and UBQ10::mCitrine-IMA1 with different treatments. The differentially expressed genes in response to +Fe, +Fe+flg22, -Fe, -Fe+flg22 were analyzed. It has 36 samples in total, with 3 biogical replicates for each condition and each genotype.

2023-10-09 | GSE213557 | GEO

Differential expression for P and Fe

Project description:Root samples were used We exposed young seedlings to P and Fe deficiency Expression study after 7 days of treatment

2016-07-03 | E-GEOD-52159 | biostudies-arrayexpress

Fe-FIT-Diff

Project description:au13-06_fit - Fe-FIT-Diff - FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR) is a regulator of Fe deficiency responses in the root. FIT is a basic helix-loop-helix protein. Here, we investigated the transcriptome changes in response to Fe deficiency (- Fe) versus the control condition (+ Fe) in wild type, the fit-3 loss of function mutant and in FIT overexpression plants.

2015-02-14 | GSE65934 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data